Detailed information of TA system TA162306

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 132670..132923
• Replicon: plasmid pKP55_2
• Accession: NZ_CP055296
• Organism: Klebsiella pneumoniae strain KP55

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUW33_RS28860
• Protein ID: WP_001312851.1
• Coordinates: 132774..132923 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	132670..132729 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW33_RS28820 (128052)	128052..128396	-	345	Protein_162	IS6-like element IS26 family transposase	-
HUW33_RS28825 (128448)	128448..129152	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HUW33_RS28830 (129177)	129177..129377	+	201	WP_072354025.1	hypothetical protein	-
HUW33_RS28835 (129397)	129397..130143	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
HUW33_RS28840 (130198)	130198..130758	+	561	WP_000139328.1	fertility inhibition protein FinO	-
HUW33_RS28845 (130890)	130890..131090	+	201	WP_015059022.1	hypothetical protein	-
HUW33_RS28850 (131476)	131476..132075	+	600	WP_032083981.1	PIN domain-containing protein	-
HUW33_RS28855 (132137)	132137..132469	+	333	WP_152916585.1	hypothetical protein	-
- (132670)	132670..132729	-	60	NuclAT_1	-	Antitoxin
- (132670)	132670..132729	-	60	NuclAT_1	-	Antitoxin
- (132670)	132670..132729	-	60	NuclAT_1	-	Antitoxin
- (132670)	132670..132729	-	60	NuclAT_1	-	Antitoxin
HUW33_RS28860 (132774)	132774..132923	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
HUW33_RS28865 (133207)	133207..133455	+	249	WP_000083837.1	replication regulatory protein RepA	-
HUW33_RS29995 (133570)	133570..133754	+	185	Protein_172	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..133766	133766

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T162306 WP_001312851.1 NZ_CP055296:132774-132923 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T162306 NZ_CP071730:c595781-595026 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATGCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAACTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT162306 NZ_CP055296:c132729-132670 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin