Detailed information of TA system TA162243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1801864..1802466
• Replicon: chromosome
• Accession: NZ_CP055292
• Organism: Shigella sonnei strain SE6-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUX90_RS08495
• Protein ID: WP_000897305.1
• Coordinates: 1802155..1802466 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUX90_RS08490
• Protein ID: WP_000356397.1
• Coordinates: 1801864..1802154 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUX90_RS08465	1797789..1798691	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HUX90_RS08470	1798688..1799323	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUX90_RS08475	1799320..1800249	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HUX90_RS08480	1800579..1800821	-	243	WP_001087409.1	hypothetical protein	-
HUX90_RS08485	1801041..1801259	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
HUX90_RS08490	1801864..1802154	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HUX90_RS08495	1802155..1802466	-	312	WP_000897305.1	hypothetical protein	Toxin
HUX90_RS08500	1802695..1803603	+	909	WP_001162704.1	alpha/beta hydrolase	-
HUX90_RS08505	1803667..1804608	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HUX90_RS08510	1804653..1805090	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HUX90_RS08515	1805087..1805959	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HUX90_RS08520	1805953..1806552	-	600	WP_001295269.1	glucose-1-phosphatase	-
HUX90_RS08525	1806651..1807436	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T162243 WP_000897305.1 NZ_CP055292:c1802466-1802155 [Shigella sonnei]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T162243 NZ_CP071695:c23179-22898 [Salmonella sp. SAL-045]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT162243 WP_000356397.1 NZ_CP055292:c1802154-1801864 [Shigella sonnei]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT162243 NZ_CP071695:c23420-23169 [Salmonella sp. SAL-045]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7