Detailed information of TA system TA162193

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 58991..59260
• Replicon: plasmid pAH62-3
• Accession: NZ_CP055262
• Organism: Escherichia coli strain AH62

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HU676_RS24075
• Protein ID: WP_001312861.1
• Coordinates: 59102..59260 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	58991..59056 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU676_RS24045	54543..56444	+	1902	WP_058914895.1	group II intron reverse transcriptase/maturase	-
HU676_RS24050	56594..57091	+	498	WP_042039995.1	single-stranded DNA-binding protein	-
HU676_RS24055	57159..57392	+	234	WP_000005990.1	DUF905 family protein	-
HU676_RS24060	57420..57617	+	198	Protein_47	hypothetical protein	-
HU676_RS24065	57672..58106	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
HU676_RS24070	58103..58852	+	750	WP_072648274.1	plasmid SOS inhibition protein A	-
-	58834..59058	+	225	NuclAT_0	-	-
-	58834..59058	+	225	NuclAT_0	-	-
-	58834..59058	+	225	NuclAT_0	-	-
-	58834..59058	+	225	NuclAT_0	-	-
-	58991..59056	+	66	-	-	Antitoxin
HU676_RS24075	59102..59260	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HU676_RS24080	60181..60468	+	288	WP_000107535.1	hypothetical protein	-
HU676_RS24085	60586..61407	+	822	WP_001234426.1	DUF945 domain-containing protein	-
HU676_RS24090	61704..62306	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HU676_RS24095	62627..63010	+	384	WP_001151524.1	relaxosome protein TraM	-
HU676_RS24100	63197..63886	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
HU676_RS24105	63985..64140	+	156	Protein_56	TraY domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sitABCD / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / aac(3)-IId / aadA5 / qacE / sul1 / mph(A)	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..136694	136694
	-	flank	IS/Tn	-	-	64153..64809	656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T162193 WP_001312861.1 NZ_CP055262:59102-59260 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T162193 NZ_CP071686:836168-836566 [Salmonella sp. SAL-007]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT162193 NZ_CP055262:58991-59056 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin