Detailed information of TA system TA162188

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39433..39697
• Replicon: plasmid pAH62-2
• Accession: NZ_CP055261
• Organism: Escherichia coli strain AH62

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: HU676_RS23600
• Protein ID: WP_001331364.1
• Coordinates: 39545..39697 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	39433..39490 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU676_RS23585	35771..36841	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
HU676_RS23590	36860..38068	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
HU676_RS23595	38286..39272	-	987	WP_001257838.1	hypothetical protein	-
-	39433..39490	-	58	NuclAT_0	-	Antitoxin
-	39433..39490	-	58	NuclAT_0	-	Antitoxin
-	39433..39490	-	58	NuclAT_0	-	Antitoxin
-	39433..39490	-	58	NuclAT_0	-	Antitoxin
HU676_RS23600	39545..39697	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
HU676_RS23605	39769..40020	-	252	WP_001291964.1	hypothetical protein	-
HU676_RS23610	40679..40855	-	177	WP_001054901.1	hypothetical protein	-
HU676_RS23615	41187..41396	+	210	WP_000062602.1	HEAT repeat domain-containing protein	-
HU676_RS23620	41468..42130	-	663	WP_060527638.1	plasmid IncI1-type surface exclusion protein ExcA	-
HU676_RS23625	42201..44369	-	2169	WP_000698368.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..84033	84033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T162188 WP_001331364.1 NZ_CP055261:39545-39697 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T162188 NZ_CP071684:c1784282-1783791 [Bifidobacterium longum subsp. longum]
ATGAGGCAGTCAAAGCAGGTGTCGATCTTGTGCGACACCAATATCCTTATTTATTACATGCTCTGTACGCCTCGGTTCAT
CAAGGATGTCATGAAACTGTTCGAGCGATGCGTCGAGCGAGAGGTAAAGCTGTCCTGTGCGTCACTGTCGCTGAAGGACG
TGTTCTACATCACCCCGGAACGATTCAAAGAACGATTCATGAATGACGAATCCATGGTCGTTGCGACCACCGCCAAGCTG
ATATTGCCGGAGGTGGCATGGTCGGGAATCAGCAAACTCAAGGAGCTCATGACAATCGTGCCGATTGGCGCGGAGGAATG
TGAATTGGCGCAGTCGTTGCGGCACGCGCATAACGATTTCGAGGATGACCTTATTATCGCCGCCGCACAACAATCCGGGG
CCACCTATGTGGTGACGTATGACAAAAAACTAGCCAAGCATTTTCCCGAATATTGCAAAACGCCGGCTGAAATCGTGGCG
GCGCTGGCATAG

Antitoxin

Nucleotide

• Length: 58 bp

>AT162188 NZ_CP055261:c39490-39433 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin