Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3740092..3740633 Replicon chromosome
Accession NZ_CP055259
Organism Escherichia coli strain AH62

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HU676_RS18090 Protein ID WP_000615976.1
Coordinates 3740355..3740633 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HU676_RS18085 Protein ID WP_000729704.1
Coordinates 3740092..3740352 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HU676_RS18065 3735697..3736482 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
HU676_RS18070 3736427..3738166 + 1740 WP_001325255.1 flagellar biosynthesis protein FlhA -
HU676_RS18075 3738460..3738957 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HU676_RS18080 3739133..3739906 - 774 WP_015675129.1 C40 family peptidase -
HU676_RS18085 3740092..3740352 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HU676_RS18090 3740355..3740633 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HU676_RS18095 3740789..3741529 + 741 WP_001225679.1 murein L,D-transpeptidase -
HU676_RS18100 3741500..3742267 - 768 WP_000333380.1 class II glutamine amidotransferase -
HU676_RS18105 3742473..3743051 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T162181 WP_000615976.1 NZ_CP055259:3740355-3740633 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T162181 NZ_CP071679:c61673-61245 [Rhizobium ruizarguesonis]
GTGAGACTTCTGCTGGACACCAATGTGCTTTCCGAGGTGACAAGGCCGCGTCCCGATGCACATGTCCTGCAATGGCTGGA
CGATCTCGACGAGGATCGCTCATTCATCAGCGTCGTATCGATCGCGGAGATCCGGCGTGGTGTTGCATTGATGGAAAGCG
GTCGGAAGCGCGACGCGCTGGCTGAATGGCTTGCCCAGGACCTGCCGCAACGCTTCGAGCAGCGTGTCATCCCTGTAGAC
CAGGCGGTTGCGATAACCTCGGGAGACTTGATGGGGCTCGCAAAGCGAAGCGGACGCGGCTTGTCGTCAATGGGTGGCTT
GATTGCCGCCACCGCGATTGCCCACGATCTCACCCTCGCCACCCGTAATATAAAGGATTTCGAGAGCCTTGGGATCGAGC
TTGTCGATCCTTGGATCGAGCGCCCGTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT162181 WP_000729704.1 NZ_CP055259:3740092-3740352 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT162181 NZ_CP071679:c61936-61670 [Rhizobium ruizarguesonis]
ATGCAGCCATCCCGACCAGAGGATAAAAGTTGGACCGTCGCCAGTGCGAAGGCCAAGCTGTCTGAGGTTATCGAACGCGC
GCAAGCAACACCGCAAACGATCATGCGCAATGGTAAACCAAGCGTCGTCGTCGTTTCGGCCGAGGAATGGCAGCGGAAGA
CCGCGCGCAAGGGCACCCTTGCAGAGTTTCTGATGCAATCGCCGCTGCGTGGCGCCGATCTGGACCTCGAACGGCAGCGC
GACGAACCGCGTGATCTCCAGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References