Detailed information of TA system TA162180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3733825..3734519
• Replicon: chromosome
• Accession: NZ_CP055259
• Organism: Escherichia coli strain AH62

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HU676_RS18050
• Protein ID: WP_001263493.1
• Coordinates: 3733825..3734223 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HU676_RS18055
• Protein ID: WP_000554757.1
• Coordinates: 3734226..3734519 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU676_RS18020	3728825..3730069	-	1245	WP_000189541.1	esterase FrsA	-
-	3729485..3729565	-	81	NuclAT_9	-	-
-	3729485..3729565	-	81	NuclAT_9	-	-
-	3729485..3729565	-	81	NuclAT_9	-	-
-	3729485..3729565	-	81	NuclAT_9	-	-
HU676_RS18025	3730161..3730619	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HU676_RS18030	3730880..3732337	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HU676_RS18035	3732394..3732951	-	558	WP_001353768.1	peptide chain release factor H	-
HU676_RS18040	3732863..3733129	-	267	WP_001321003.1	hypothetical protein	-
HU676_RS18045	3733363..3733815	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HU676_RS18050	3733825..3734223	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HU676_RS18055	3734226..3734519	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HU676_RS18060	3734571..3735626	-	1056	WP_001226164.1	DNA polymerase IV	-
HU676_RS18065	3735697..3736482	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
HU676_RS18070	3736427..3738166	+	1740	WP_001325255.1	flagellar biosynthesis protein FlhA	-
HU676_RS18075	3738460..3738957	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T162180 WP_001263493.1 NZ_CP055259:c3734223-3733825 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T162180 NZ_CP055259:c3734223-3733825 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT162180 WP_000554757.1 NZ_CP055259:c3734519-3734226 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT162180 NZ_CP055259:c3734519-3734226 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6