Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 658491..659218 Replicon chromosome
Accession NZ_CP055259
Organism Escherichia coli strain AH62

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HU676_RS03175 Protein ID WP_000550189.1
Coordinates 658491..658805 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HU676_RS03180 Protein ID WP_000560266.1
Coordinates 658802..659218 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HU676_RS03155 654649..655635 - 987 WP_176256439.1 Gfo/Idh/MocA family oxidoreductase -
HU676_RS03160 655714..656406 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HU676_RS03165 656483..656986 - 504 WP_001300832.1 M48 family metallopeptidase -
HU676_RS03170 657071..658207 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HU676_RS03175 658491..658805 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HU676_RS03180 658802..659218 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HU676_RS03185 659263..661281 - 2019 WP_000121453.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HU676_RS03190 661707..664058 - 2352 WP_000695495.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T162164 WP_000550189.1 NZ_CP055259:658491-658805 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T162164 NZ_CP071626:c3711860-3711555 [Rhizobium leguminosarum]
ATGTTCATCTTGAAGCAGACAGCTACTTTTCGAAAGTGGCACAAAAAATTGAAAGATGGAAAGGCCAAGGCGACGATTGC
CTTGCGTCTTCAGCGTCTCGCGACCGGGCATGCCGGCGACGCCTCACCGATCGGCGAGGGGCTGAGCGAGTTGCGCATTC
ACTATGGGCCTGGCTATCGGGTCTATTATTGTAAACGCGGAGAAACTATCATCGTGCTTCTCTGCGGCGGTGACAAAAGC
ACGCAGGAAACCGATATACGAACGGCAAAAAGAATCGCCTCGGAATGGAGCGACGATAATGGCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT162164 WP_000560266.1 NZ_CP055259:658802-659218 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT162164 NZ_CP071626:c3711562-3711272 [Rhizobium leguminosarum]
ATGGCTGAACAGATTTTCGACTTTGATCCGGCTGAGATGCTTGATTCCGACGAGGCCATCGAAGCCTTTATCATCGAGGC
ATTGCAGACGGGAGACGCGAAATTCATCGCGTCCGCTCTTGGTGTCGTCGCGCGTGCCAAGGGCATGAGTAAGATCTCCA
GGGAAACAGGCCTTGCTCGCGAGCATCTTTATAAGGCACTGAGCGAAAACGGAAATCCCACCCTGGAGACCACGCTGGCG
GTCATGAAGGCAATCGGGCTTGAGCTGACGACCAAGCATCACGCGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References