Detailed information of TA system TA162159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4938515..4939117
• Replicon: chromosome
• Accession: NZ_CP055256
• Organism: Escherichia coli strain AH25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUU84_RS23710
• Protein ID: WP_000897305.1
• Coordinates: 4938806..4939117 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUU84_RS23705
• Protein ID: WP_000356397.1
• Coordinates: 4938515..4938805 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU84_RS23680	4934460..4935362	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HUU84_RS23685	4935359..4935994	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUU84_RS23690	4935991..4936920	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HUU84_RS23695	4937250..4937492	-	243	WP_001086388.1	hypothetical protein	-
HUU84_RS23700	4937711..4937929	-	219	WP_001611167.1	ribbon-helix-helix domain-containing protein	-
HUU84_RS23705	4938515..4938805	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HUU84_RS23710	4938806..4939117	-	312	WP_000897305.1	hypothetical protein	Toxin
HUU84_RS23715	4939346..4940254	+	909	WP_001162704.1	alpha/beta hydrolase	-
HUU84_RS23720	4940318..4941259	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HUU84_RS23725	4941304..4941741	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HUU84_RS23730	4941738..4942610	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HUU84_RS23735	4942604..4943203	-	600	WP_001295269.1	glucose-1-phosphatase	-
HUU84_RS23740	4943302..4944087	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T162159 WP_000897305.1 NZ_CP055256:c4939117-4938806 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T162159 NZ_CP071626:c1310243-1308981 [Rhizobium leguminosarum]
ATGACATCAAGGCTTCTCAACGTCTGGTGGGACGGCCGCATTGTCGGTCAATTCACGCAGGACAGACACGGCGATATCGG
CTTTGTCTATGCGCGCGCCTGGCTTGACGATGACAAAACGCTGCCACTCTCGGCCTCTCTGCCGAAACGACCGGAGCGAT
TTACCCGCCAGGAATGCCGGCCGTTTTTCGCCGGCCTGCTGCCCGAGGAAAGCCAACGCCTGGCCGCGGCACGGGCCTTG
GGCGTGTCTCCCGCCAATGACTTTGCGCTTCTCGATCACCTGGGCGGCGATGTCGCCGGTGCGCTGCAACTGCTTGCCGA
AGGTGAGGCACCGGCCGAGACAGCGTCGATTGCAGATCGCCAGCCCGTGCCCTTGGATGAAGCAGGCATCGTTGGCGTGT
TGGAGGCACTGCCCGTGCGGCCTCTCCTGGCCGGTGAGGAGGGCCTCAGACTGTCGCTTGCCGGCGCGCAATCCAAACTG
CCGGTTGTTCTCATCGATGGCCGGATCGGGCTTCCCCTGCCCGGTCAGGCCACAACCCATATTCTGAAGCCACCGATAGC
CCGCTTCAAGGCGACGACCGAGAACGAAGCTTTCGTGATGAGGCTTGCCGCCGCCATCGGGCTCGATGTCGCAGCCGTCG
AACCGAGATATGTGCAGGGACGCCCGTTCCTTCTGGTCGAACGATATGACCGCGTGCGCGGCGCCGGCGGCCTGGTTCAT
CGCATCCATCAGGAAGATTTCTGCCAGGCACTCAGCGTGCCGCCGGAAACGAAATATGCGAGTGAAGGCGGGCCGACCTT
CAAGGATTGCTTCGATCTCATAAGACGGATCTCGGCAAGGCCGGCTGTCGACGTGCTGAAGCTGCTGGACGCGGCCATCT
TCAACCTGATCGCCGGAAATGCCGATGCCCATGGCAAGAACTTCTCCATCCTGTACGATAGCCAAGGCCCGCGGCTGGCA
AGACTCTATGACCTCCTCTCCACCGTGATCTATCCGGAACTGTCGCCAAGGCTGGCGATGAGACTAGGCAAGCGCGCCAC
ACTGGCCGAAATGGATGCCGAGGGCTGGAAGGCCTTTGCGGCAGAAGCCGGCGTCGGCACTCCGCTGTTGCGCCGCCGCC
TGAACGAGCTTTGCGACAAGGTGCTGACATTGACCGGCAAGGTCGCCGATGAGCTGGCAGACGTATCGCCCGATCGAGGC
CCGATCGACGCGATCGCCGGCGTGATAACAGACCGGGCCGAGACAGCGAGGTTGACTTTATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT162159 WP_000356397.1 NZ_CP055256:c4938805-4938515 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT162159 NZ_CP071626:c1310470-1310240 [Rhizobium leguminosarum]
ATGCCAAAACACGTACAAAGTCCAGCCGACATCGGCGCCCTGGTTCGAAGCACCCGCAAGGAGCAAAACCTGCGGCAGGA
CGAGCTTGCTGGCGTTGCCGGCGTCGGGCTTCGCTTTATCGTCGATCTCGAAGCCGGCAAGCCCACAGCGCAGATCGGCA
AAGTCCTGCAGGTTCTGCAAACGCTTGGCTGCTCCCTCGACATTCTGGCTCCCGGCGAGCGCAGGCAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7