Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3979576..3980117 Replicon chromosome
Accession NZ_CP055256
Organism Escherichia coli strain AH25

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag HUU84_RS19295 Protein ID WP_000615982.1
Coordinates 3979839..3980117 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUU84_RS19290 Protein ID WP_000729704.1
Coordinates 3979576..3979836 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU84_RS19265 3975359..3976144 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
HUU84_RS19270 3976089..3977828 + 1740 WP_001298878.1 flagellar biosynthesis protein FlhA -
HUU84_RS19275 3977933..3978211 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HUU84_RS19280 3978204..3978560 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HUU84_RS19285 3978617..3979390 - 774 WP_000543899.1 C40 family peptidase -
HUU84_RS19290 3979576..3979836 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUU84_RS19295 3979839..3980117 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUU84_RS19300 3980273..3981013 + 741 WP_001225679.1 murein L,D-transpeptidase -
HUU84_RS19305 3980984..3981751 - 768 WP_000333380.1 class II glutamine amidotransferase -
HUU84_RS19310 3981957..3982535 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T162154 WP_000615982.1 NZ_CP055256:3979839-3980117 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T162154 NZ_CP071621:c334174-333755 [Rhizobium bangladeshense]
ATGGCGCGTTACATGCTCGATACGAACATGTGTATCTACCTGATGAAAAACCAGCCGGAGCAGGTCGCTCGCCGCTTTGC
CAGTTGCTATGTGGGGGACGTGGTGATGTCCGCCATCACCTTCGCGGAACTCGGGTACGGTGTTTCCGCATCCGATGATC
CGGCACGAGAACTTGATAATCTCACCGCCCTGGCCGAGCTGATCACGGTAGAGCCGTTTGGGATCGCAGCAGCGCGTGCC
TACGGTCCGGTTCGAATGGCGACGAGAGACCGGAAGATAGATCATCTCGACAAACTGATCGCATCGCATGCGATCGCTCT
GGACACGGTATTGGTGACCAACAACACGCGGGACTTCCTGGCGCATCCCGGACTGAAGCTCGAAAACTGGCTGGACGCGG
AACGCAGAGAAACCAATTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT162154 WP_000729704.1 NZ_CP055256:3979576-3979836 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT162154 NZ_CP071621:c334410-334174 [Rhizobium bangladeshense]
ATGCACACCACTAAAGCTTTCAAGAATGGAAATTCCCAGGCGGTTCGCATACCGGCGGAACTGGCGTTTGAGCGAACCGA
TGTTGAGTTGGAAATCGAGCGCATCGGCGACGAGCTTCGCATCCGTCCTGTGCATCGCTCCCTGGCCGGCGTTCTGGATG
TATTCGCCCGCTTCTCTCCGGATTTCATGCGCGACGGAAGAGAGTCCCATGAGCAAGAGGAGCGGGATAAGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References