Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 636109..636908 Replicon chromosome
Accession NZ_CP055256
Organism Escherichia coli strain AH25

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag HUU84_RS03130 Protein ID WP_000347266.1
Coordinates 636109..636573 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HUU84_RS03135 Protein ID WP_001307405.1
Coordinates 636573..636908 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU84_RS03100 631110..631544 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HUU84_RS03105 631562..632440 - 879 WP_001298758.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HUU84_RS03110 632430..633209 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HUU84_RS03115 633220..633693 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUU84_RS03120 633716..634996 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUU84_RS03125 635245..636054 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUU84_RS03130 636109..636573 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUU84_RS03135 636573..636908 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUU84_RS03140 637057..638628 - 1572 WP_001273741.1 galactarate dehydratase -
HUU84_RS03145 639003..640337 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HUU84_RS03150 640353..641123 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T162134 WP_000347266.1 NZ_CP055256:c636573-636109 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T162134 NZ_CP071594:2831153-2831614 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTACTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGTTTATCAGATAGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCGCATAATAAACTTACATACGGTGATATAGCCGATTACTCGAATTTCAACAATCGCAAGTTCGAAAATTACGCAAGA
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTTGAAGCGCACAATTACGGTGTACGTAATTTATATGAGTT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCTATTACATTTGAGCCGTTGAGAGTTTTTAAATATAAGGAAATATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT162134 WP_001307405.1 NZ_CP055256:c636908-636573 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT162134 NZ_CP071594:2830817-2831140 [Staphylococcus aureus]
ATGAGAACTTCAGCGGAAATAGGTAAATTAATCAAACAACTACGAAAAGAGAATAATGTGAATTTAACTGATTTTGCAAC
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATATGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAGA
TTGCCAATGCATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAAACAGAAAACGAAGTACAACATCGTGCA
GCTCATTTAGAAGGAGAATTAACTGATGACGAGTGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAA
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References