Detailed information of TA system TA162129

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 120402..120828
• Replicon: plasmid pAH01-4
• Accession: NZ_CP055255
• Organism: Escherichia coli strain AH01

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HUU83_RS25360
• Protein ID: WP_001312861.1
• Coordinates: 120402..120560 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	120604..120828 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU83_RS25335	115776..116465	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
HUU83_RS25340	116652..117035	-	384	WP_001151524.1	relaxosome protein TraM	-
HUU83_RS25345	117356..117958	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HUU83_RS25350	118255..119076	-	822	WP_001234426.1	DUF945 domain-containing protein	-
HUU83_RS25355	119194..119481	-	288	WP_063121090.1	hypothetical protein	-
HUU83_RS25360	120402..120560	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	120604..120828	-	225	NuclAT_0	-	Antitoxin
-	120604..120828	-	225	NuclAT_0	-	Antitoxin
-	120604..120828	-	225	NuclAT_0	-	Antitoxin
-	120604..120828	-	225	NuclAT_0	-	Antitoxin
HUU83_RS25365	120640..120828	+	189	WP_001299721.1	hypothetical protein	-
HUU83_RS25370	120840..121559	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
HUU83_RS25375	121556..121990	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
HUU83_RS25380	122045..122242	-	198	Protein_136	hypothetical protein	-
HUU83_RS25385	122270..122503	-	234	WP_000005987.1	DUF905 family protein	-
HUU83_RS25390	122571..123110	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
HUU83_RS25395	123136..123342	-	207	WP_000275856.1	hypothetical protein	-
HUU83_RS25400	124438..125409	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..145790	145790

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T162129 WP_001312861.1 NZ_CP055255:c120560-120402 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T162129 NZ_CP071594:c470295-470188 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT162129 NZ_CP055255:c120828-120604 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin