Detailed information of TA system TA162124

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 82073..82327
• Replicon: plasmid pAH01-4
• Accession: NZ_CP055255
• Organism: Escherichia coli strain AH01

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HUU83_RS25140
• Protein ID: WP_001351576.1
• Coordinates: 82073..82279 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	82266..82327 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU83_RS25100	77625..78026	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HUU83_RS25105	77959..78216	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HUU83_RS25110	78309..78962	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HUU83_RS25115	79060..79200	-	141	WP_001333237.1	hypothetical protein	-
HUU83_RS25120	79901..80758	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
HUU83_RS25125	80751..81233	-	483	WP_001273588.1	hypothetical protein	-
HUU83_RS25130	81226..81300	-	75	WP_001442103.1	RepA leader peptide Tap	-
HUU83_RS25135	81532..81789	-	258	WP_000083845.1	replication regulatory protein RepA	-
HUU83_RS25140	82073..82279	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	82266..82327	+	62	NuclAT_1	-	Antitoxin
-	82266..82327	+	62	NuclAT_1	-	Antitoxin
-	82266..82327	+	62	NuclAT_1	-	Antitoxin
-	82266..82327	+	62	NuclAT_1	-	Antitoxin
HUU83_RS25145	82583..82657	-	75	Protein_89	endonuclease	-
HUU83_RS25150	82903..83115	-	213	WP_001299730.1	ANR family transcriptional regulator	-
HUU83_RS25155	83251..83811	-	561	WP_000139315.1	fertility inhibition protein FinO	-
HUU83_RS25160	83914..84774	-	861	WP_000704514.1	alpha/beta hydrolase	-
HUU83_RS25165	84833..85579	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..145790	145790

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T162124 WP_001351576.1 NZ_CP055255:c82279-82073 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T162124 NZ_CP071590:1870073-1870564 [Bifidobacterium longum subsp. longum]
ATGACCGACGTTTCCATGCAGCGTATTTTGCTCGATACCAACGTACTGTTGGACTATTTGCTCCATCGCGATGATCACGC
CAAGATGGCGGAAGCGGTTATGGAACTTGGCGCGAAGAACAACATTACGTTGTTGTGCGCGTCATTGTCGCTGAAGGATA
TCGCATACCTATCATCATCGGCGATTCGTAGGGAATTCAAACCGAATGAATCGGAAGTGGAGAATTTCACTCGCGGCTTT
CTGTCGTCACGGGTGCCATGGCGTTGTATCGAGCAGGTGAAGGAGATGTGTGACATCGTCGCTGTCGATGAGTCGACATG
TGATAAGGCTTTTTCCCTGCAAAAACGGCATCGGGATTTTGAAGATGATCTGATCATCGTTGCCGCGCAACAATCCGGTG
CCAACTGTGTGGTCACATCGGATGCCGAACTAATCAGTCATTTTCCCGAATATTGCAAAACACCGGCTGAAATCGTGGCG
GCACTGGAATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT162124 NZ_CP055255:82266-82327 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin