Detailed information of TA system TA162122

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70841..71094
• Replicon: plasmid pAH01-3
• Accession: NZ_CP055254
• Organism: Escherichia coli strain AH01

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUU83_RS24690
• Protein ID: WP_001312851.1
• Coordinates: 70945..71094 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	70841..70900 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU83_RS24665	67568..68314	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
HUU83_RS24670	68369..68929	+	561	WP_000139328.1	fertility inhibition protein FinO	-
HUU83_RS24675	69061..69261	+	201	WP_015059022.1	hypothetical protein	-
HUU83_RS24680	69647..70246	+	600	WP_032083981.1	hypothetical protein	-
HUU83_RS24685	70410..70640	+	231	WP_001736714.1	hypothetical protein	-
-	70841..70900	-	60	NuclAT_1	-	Antitoxin
-	70841..70900	-	60	NuclAT_1	-	Antitoxin
-	70841..70900	-	60	NuclAT_1	-	Antitoxin
-	70841..70900	-	60	NuclAT_1	-	Antitoxin
HUU83_RS24690	70945..71094	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUU83_RS24695	71378..71626	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / blaTEM-1B / blaCTX-M-55	-	1..71937	71937

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T162122 WP_001312851.1 NZ_CP055254:70945-71094 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T162122 NZ_CP071589:c2405206-2404871 [Staphylococcus simiae]
ATGAGTGTTAAGCAATTCGACATTTTATACATCGACCTAGACCCAACAAAAGGTCGCGAAAAGCAAAAGATACAACCATG
TTTAGTCATCAACAACGATATGACAATTAAAGGCACAAATTTTGTGTGGATCTTGCCAATCACAAGTCGTGAACCGAAAT
TTCCAACCGATATAGAAGTTAAATCTAAAAAGCGATTAGTTACAGGTGTGATTGATACTGTACAAATACGCGCGTTAAAT
CTAAATGCACACGAGTATAATTATAGAGACGAGTTACAAGACAGCTTGAAAAACGACGTCTTACTAGCCATCAATACTTT
TCTAAAACCACAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT162122 NZ_CP055254:c70900-70841 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin