Detailed information of TA system TA162119

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30045..30314
• Replicon: plasmid pAH01-3
• Accession: NZ_CP055254
• Organism: Escherichia coli strain AH01

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HUU83_RS24450
• Protein ID: WP_001312861.1
• Coordinates: 30156..30314 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30045..30110 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU83_RS24420	25755..26282	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HUU83_RS24425	26340..26573	+	234	WP_000006003.1	DUF905 family protein	-
HUU83_RS24430	26634..28657	+	2024	Protein_39	ParB/RepB/Spo0J family partition protein	-
HUU83_RS24435	28726..29160	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HUU83_RS24440	29157..29876	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	29888..30112	+	225	NuclAT_0	-	-
-	29888..30112	+	225	NuclAT_0	-	-
-	29888..30112	+	225	NuclAT_0	-	-
-	29888..30112	+	225	NuclAT_0	-	-
HUU83_RS24445	29897..30076	-	180	WP_001309233.1	hypothetical protein	-
-	30045..30110	+	66	-	-	Antitoxin
HUU83_RS24450	30156..30314	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUU83_RS24455	30552..30929	-	378	Protein_44	hypothetical protein	-
HUU83_RS24460	31229..31525	+	297	WP_001272251.1	hypothetical protein	-
HUU83_RS24465	31636..32457	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HUU83_RS24470	32754..33401	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
HUU83_RS24475	33678..34061	+	384	WP_000124981.1	relaxosome protein TraM	-
HUU83_RS24480	34252..34710	+	459	Protein_49	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / blaTEM-1B / blaCTX-M-55	-	1..71937	71937

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T162119 WP_001312861.1 NZ_CP055254:30156-30314 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T162119 NZ_CP071589:c1819398-1819243 [Staphylococcus simiae]
ATGGTTAATGAAAAAGATGCTAAATTAAATTATCATGAAGATGAAAATGCAATGGTAAATGATTTTGATGATTTGAAAGA
ACTAGGTAAAGAAATGGAACAAATTTCTGAAGAAAACGATCAAGAAAAGCATAATCAATCACACGATAATAATTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT162119 NZ_CP055254:30045-30110 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin