Detailed information of TA system TA162114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4854516..4855118
• Replicon: chromosome
• Accession: NZ_CP055251
• Organism: Escherichia coli strain AH01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUU83_RS23385
• Protein ID: WP_000897305.1
• Coordinates: 4854516..4854827 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUU83_RS23390
• Protein ID: WP_000356397.1
• Coordinates: 4854828..4855118 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU83_RS23355	4849546..4850331	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
HUU83_RS23360	4850430..4851029	+	600	WP_124783521.1	glucose-1-phosphatase	-
HUU83_RS23365	4851023..4851895	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HUU83_RS23370	4851892..4852329	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HUU83_RS23375	4852374..4853315	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
HUU83_RS23380	4853379..4854287	-	909	WP_001162704.1	alpha/beta hydrolase	-
HUU83_RS23385	4854516..4854827	+	312	WP_000897305.1	hypothetical protein	Toxin
HUU83_RS23390	4854828..4855118	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HUU83_RS23395	4855723..4855941	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
HUU83_RS23400	4856160..4856402	+	243	WP_001086388.1	hypothetical protein	-
HUU83_RS23405	4856732..4857661	-	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
HUU83_RS23410	4857658..4858293	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUU83_RS23415	4858290..4859192	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T162114 WP_000897305.1 NZ_CP055251:4854516-4854827 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T162114 NZ_CP071586:c3119268-3119047 [Pseudomonas germanica]
ATTGAACAATACAATTCCAACGCTTCAGCGGCTCTGCAGCGCAAAGTGGGTGCAGCAACGCAAAGGCTTTCATCAATTCC
CTATGGTCACAGATCCGGTCGAGTACCAGGCACTCGGGAAATGGTGGTCAATCCAAACTATCTACTGGTCTATCGAGTGA
ACGGGCGCATCAAAATCCTGACGTTAGTCCACACCCGACAACAATACCCACGAACCTCATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT162114 WP_000356397.1 NZ_CP055251:4854828-4855118 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT162114 NZ_CP071586:c3119509-3119315 [Pseudomonas germanica]
ATGAGCATATTGCTTTCCCCGTTTTATTCTGAGTTCGAATCCGAAGAAGAGGCAGAGAGCTACGATCGCTGGTTTCGTGC
CAAGGTGCAGGCTGCACTGGATGATCCTCGCCCAGGCATTCCCCATGAGGAAGCCATGGTGCGGCTCGACCAACTATTAG
AAGAGAAACGCAAGAATCGACGCACTGCCGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7