Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2828104..2828606 Replicon chromosome
Accession NZ_CP055251
Organism Escherichia coli strain AH01

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HUU83_RS13790 Protein ID WP_000767829.1
Coordinates 2828104..2828358 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HUU83_RS13795 Protein ID WP_001259255.1
Coordinates 2828355..2828606 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU83_RS13760 2823119..2823346 - 228 WP_000234896.1 sulfurtransferase TusA -
HUU83_RS13765 2823360..2824418 - 1059 Protein_2689 transport protein YeeE -
HUU83_RS13770 2824597..2825955 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HUU83_RS13775 2825945..2826007 - 63 WP_010723108.1 membrane protein YoeI -
HUU83_RS13780 2826222..2827151 - 930 WP_000803366.1 LysR family transcriptional regulator -
HUU83_RS13785 2827197..2828021 - 825 WP_000754752.1 SDR family oxidoreductase -
HUU83_RS13790 2828104..2828358 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HUU83_RS13795 2828355..2828606 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HUU83_RS13800 2828890..2828940 + 51 WP_001364200.1 his operon leader peptide -
HUU83_RS13805 2829086..2829985 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HUU83_RS13810 2829991..2831295 + 1305 WP_039023537.1 histidinol dehydrogenase -
HUU83_RS13815 2831292..2832362 + 1071 WP_039023536.1 histidinol-phosphate transaminase -
HUU83_RS13820 2832362..2833429 + 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T162107 WP_000767829.1 NZ_CP055251:c2828358-2828104 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T162107 NZ_CP071523:48705-48863 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT162107 WP_001259255.1 NZ_CP055251:c2828606-2828355 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT162107 NZ_CP071523:48441-48640 [Escherichia coli]
TCACAACGGATTGCCCGTAAACAGCCTGAATGAGCGAGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATAT
GGGCTATGATGTGCCCGGCGCTTGAGGTTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGG
ACAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References