Detailed information of TA system TA162093

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2088147..2088518
• Replicon: chromosome
• Accession: NZ_CP055251
• Organism: Escherichia coli strain AH01

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: HUU83_RS10135
• Protein ID: WP_001317028.1
• Coordinates: 2088324..2088518 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2088147..2088325 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU83_RS10110	2084102..2085475	+	1374	WP_124783509.1	ATP-dependent RNA helicase DbpA	-
HUU83_RS10115	2085604..2086539	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
HUU83_RS10120	2086591..2087826	-	1236	WP_000040858.1	site-specific integrase	-
HUU83_RS10125	2087828..2088043	-	216	WP_000079604.1	excisionase XisR	-
HUU83_RS10130	2088122..2088331	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2088147..2088325	+	179	NuclAT_0	-	Antitoxin
-	2088147..2088325	+	179	NuclAT_0	-	Antitoxin
-	2088147..2088325	+	179	NuclAT_0	-	Antitoxin
-	2088147..2088325	+	179	NuclAT_0	-	Antitoxin
HUU83_RS10135	2088324..2088518	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HUU83_RS10140	2088575..2089384	-	810	WP_000166319.1	recombination protein RecT	-
HUU83_RS10145	2089377..2091968	-	2592	WP_000105142.1	exodeoxyribonuclease VIII	-
HUU83_RS10150	2092070..2092345	-	276	WP_000632297.1	protein RacC	-
HUU83_RS10155	2092420..2092590	-	171	WP_001352098.1	conserved protein, Rac prophage	-
HUU83_RS10160	2092590..2092811	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2055067..2146904	91837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T162093 WP_001317028.1 NZ_CP055251:c2088518-2088324 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T162093 NZ_CP071522:c2697045-2696943 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 179 bp

>AT162093 NZ_CP055251:2088147-2088325 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin