Detailed information of TA system TA162085

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 88576..88829
• Replicon: plasmid pZY-1
• Accession: NZ_CP055248
• Organism: Citrobacter freundii strain ZY198

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: GTH18_RS24470
• Protein ID: WP_001312851.1
• Coordinates: 88680..88829 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	88576..88635 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH18_RS24445	85303..86049	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
GTH18_RS24450	86104..86664	+	561	WP_000139328.1	fertility inhibition protein FinO	-
GTH18_RS24455	86796..86996	+	201	WP_015059022.1	hypothetical protein	-
GTH18_RS24460	87382..87981	+	600	WP_105906770.1	hypothetical protein	-
GTH18_RS24465	88145..88375	+	231	WP_001736714.1	hypothetical protein	-
-	88576..88635	-	60	NuclAT_1	-	Antitoxin
-	88576..88635	-	60	NuclAT_1	-	Antitoxin
-	88576..88635	-	60	NuclAT_1	-	Antitoxin
-	88576..88635	-	60	NuclAT_1	-	Antitoxin
GTH18_RS24470	88680..88829	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
GTH18_RS24475	89113..89361	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..89672	89672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T162085 WP_001312851.1 NZ_CP055248:88680-88829 [Citrobacter freundii]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T162085 NZ_CP071522:198454-198561 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT162085 NZ_CP055248:c88635-88576 [Citrobacter freundii]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin