Detailed information of TA system TA162081

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 49115..49385
• Replicon: plasmid pZY-1
• Accession: NZ_CP055248
• Organism: Citrobacter freundii strain ZY198

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GTH18_RS24245
• Protein ID: WP_001312861.1
• Coordinates: 49227..49385 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	49115..49178 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH18_RS24220	44826..45353	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
GTH18_RS24225	45411..45644	+	234	WP_000006003.1	DUF905 family protein	-
GTH18_RS24230	45705..47728	+	2024	Protein_58	ParB/RepB/Spo0J family partition protein	-
GTH18_RS24235	47797..48231	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
GTH18_RS24240	48228..48947	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	48959..49183	+	225	NuclAT_0	-	-
-	48959..49183	+	225	NuclAT_0	-	-
-	48959..49183	+	225	NuclAT_0	-	-
-	48959..49183	+	225	NuclAT_0	-	-
-	49115..49178	-	64	-	-	Antitoxin
GTH18_RS24245	49227..49385	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
GTH18_RS24250	50282..50578	+	297	WP_001272251.1	hypothetical protein	-
GTH18_RS24255	50689..51510	+	822	WP_001234445.1	DUF945 domain-containing protein	-
GTH18_RS24260	51807..52454	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
GTH18_RS24265	52731..53114	+	384	WP_000124981.1	relaxosome protein TraM	-
GTH18_RS24270	53305..53991	+	687	WP_015059009.1	PAS domain-containing protein	-
GTH18_RS24275	54085..54312	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / fosA3 / blaTEM-1B / blaCTX-M-55	-	1..89672	89672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T162081 WP_001312861.1 NZ_CP055248:49227-49385 [Citrobacter freundii]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T162081 NZ_CP071521:c4126118-4125768 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Nucleotide

• Length: 64 bp

>AT162081 NZ_CP055248:c49178-49115 [Citrobacter freundii]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin