Detailed information of TA system TA162055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6565122..6565723
• Replicon: chromosome
• Accession: NZ_CP055233
• Organism: Bradyrhizobium diazoefficiens strain 113-2

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q89QW2
• Locus tag: HUW42_RS31235
• Protein ID: WP_011085796.1
• Coordinates: 6565122..6565403 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: HUW42_RS31240
• Protein ID: WP_011085795.1
• Coordinates: 6565415..6565723 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW42_RS31200	6560505..6560867	+	363	WP_011085803.1	hypothetical protein	-
HUW42_RS31205	6560913..6561236	+	324	WP_011085802.1	antibiotic biosynthesis monooxygenase	-
HUW42_RS31210	6561259..6562125	-	867	WP_011085801.1	helix-turn-helix transcriptional regulator	-
HUW42_RS31215	6562305..6563249	+	945	WP_011085800.1	metallophosphoesterase	-
HUW42_RS31220	6563275..6563610	+	336	WP_011085799.1	cupredoxin family copper-binding protein	-
HUW42_RS31225	6563738..6564286	+	549	WP_028172321.1	sigma-70 family RNA polymerase sigma factor	-
HUW42_RS31230	6564283..6565050	+	768	WP_011085797.1	anti-sigma factor	-
HUW42_RS31235	6565122..6565403	+	282	WP_011085796.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUW42_RS31240	6565415..6565723	+	309	WP_011085795.1	HigA family addiction module antidote protein	Antitoxin
HUW42_RS31245	6565732..6567123	-	1392	WP_011085794.1	aminobacteriohopanetriol synthase HpnO	-
HUW42_RS31250	6567265..6567921	+	657	WP_038965759.1	DUF2147 domain-containing protein	-
HUW42_RS31255	6567889..6570477	-	2589	WP_011085792.1	MMPL family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10528.99 Da
• Isoelectric Point: 9.7579

>T162055 WP_011085796.1 NZ_CP055233:6565122-6565403 [Bradyrhizobium diazoefficiens]
VIRTFRDKTTEAVFDGESPKGFPADLVKVARRKLRYLHAAGELGDLRAPPGNRLEALSGNRKGQHSIRINDQFRVCFIWT
PQGPVEVEIVDYH

Nucleotide

• Length: 282 bp

>T162055 NZ_CP071512:806629-806994 [Staphylococcus haemolyticus]
ATGATTAGAAGAGGCGATGTATATTTAGCTGATTTGTCACCAGTACAGGGCTCAGAACAAGGGGGAGTCAGACCTGTAGT
TATCATTCAAAACGATACTGGCAACAAATATAGTCCTACCGTAATCGTTGCTGCGATTACGGGAAGGATTAATAAAGCGA
AAATACCCACACATGTTGAAATCGAGAAAAAGAAATACAGACTTGATAAAGATTCAGTAATTCTACTCGAGCAAATTAGA
ACATTAGATAAAAAACGACTAAAAGAAAAATTAACATATCTATCTGATGAGAAAATGCAAGAAGTAGATGAAGCACTAGA
CATTAGTCTAGGACTGCATGATGAAGTTAAAACGCAAAACACATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11266.07 Da
• Isoelectric Point: 9.0966

>AT162055 WP_011085795.1 NZ_CP055233:6565415-6565723 [Bradyrhizobium diazoefficiens]
MAKKLKPMHPGEVLREEFLVPLNMSAGALAKTCGLPRTRIERIANEQTGITADTALRLAKALGTTAELWLNLQNDYDIQI
AKRDLGKALDRIETVNRPQAAE

Nucleotide

• Length: 309 bp

>AT162055 NZ_CP071512:806462-806632 [Staphylococcus haemolyticus]
ATGCTTACTTTCAATCAAAATAGAAGTCACAGTCTTGAACAATCTTTAAAAGAAGGCTATTCACAAATGGCTGAATTAAA
TCTCTCCCTAGCGACAGAAGCTTTTCCTCTAGAATGCGAAGCTTGTGATTGCAACGAAACGTACATTATTTCTAATTATA
AAAGTGAATGA

Structures

Toxin

• AlphaFold DB: Q89QW2