Detailed information of TA system TA162023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4456957..4457681
• Replicon: chromosome
• Accession: NZ_CP055224
• Organism: Pectobacterium atrosepticum strain Green1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q6DA89
• Locus tag: HLB43_RS19710
• Protein ID: WP_011091996.1
• Coordinates: 4457370..4457681 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HLB43_RS19705
• Protein ID: WP_011091997.1
• Coordinates: 4456957..4457373 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLB43_RS19680 (HLB43_19670)	4452445..4452939	-	495	WP_039296802.1	YhbP family protein	-
HLB43_RS19685 (HLB43_19675)	4452949..4453395	-	447	WP_011092001.1	helix-turn-helix domain-containing protein	-
HLB43_RS19690 (HLB43_19680)	4453549..4454829	+	1281	WP_039296944.1	MFS transporter	-
HLB43_RS19695 (HLB43_19685)	4454863..4456215	+	1353	WP_039296941.1	pyridoxal-dependent decarboxylase	-
HLB43_RS19700 (HLB43_19690)	4456296..4456754	-	459	WP_039296801.1	hypothetical protein	-
HLB43_RS19705 (HLB43_19695)	4456957..4457373	-	417	WP_011091997.1	helix-turn-helix domain-containing protein	Antitoxin
HLB43_RS19710 (HLB43_19700)	4457370..4457681	-	312	WP_011091996.1	type II toxin-antitoxin system HigB family toxin	Toxin
HLB43_RS19715 (HLB43_19705)	4457841..4458392	-	552	WP_039294652.1	polymer-forming cytoskeletal protein	-
HLB43_RS19720 (HLB43_19710)	4458864..4459799	+	936	WP_011091994.1	aminoimidazole riboside kinase	-
HLB43_RS19725 (HLB43_19715)	4459990..4461525	+	1536	WP_011091993.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12431.33 Da
• Isoelectric Point: 10.0380

>T162023 WP_011091996.1 NZ_CP055224:c4457681-4457370 [Pectobacterium atrosepticum]
MHVISRAPFDAATNQFPNQAVALADLYRVIKREMYATPEDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T162023 NZ_CP071489:c1543538-1543254 [Glaesserella parasuis]
ATGAGCTATACCATTGAATTTATCCCATCAGCAGAAAAAGAGTTTCGTAAACTAAGCTTAGATTTAAGAAAACAATTTAT
CGAAAAACTTAAAGAAAGAGCTGAAAATCCGCGTGTAGAAAGTGCTAAACTTCGTGGAATGAAAGATTGTTATAAAATCA
AATTGCGTAATGCAGGCTATCGTTTGGTGTATCAAGTGATTGATGAACGTATTGTAATCAAAGTGGTTGCACTGGGAAAA
AGGGAGCGCAACGACGTTTACCAAAAAGCACAATTACGTTTATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15395.73 Da
• Isoelectric Point: 4.7943

>AT162023 WP_011091997.1 NZ_CP055224:c4457373-4456957 [Pectobacterium atrosepticum]
MMYADAIKAANDLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSSLIDMLTAKIDKYENEAPEFAAFNERIKALPAGI
ALLRVLMDQHQLMQSDFEAEIGKKSLVSRILNGERTLTLAHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT162023 NZ_CP071489:c1543789-1543535 [Glaesserella parasuis]
ATGCCAAGTATTATTTTAACCAATACTGTTGCGAGTATTACAGAGCTAAAGACAAATCCAATGGCGACATTCAATGCGGC
AGGCGGTGAGGCAATTGCAATTTTAAACCGAAATGAACCCGCTTTTTATTGTGTACCACCGCATATTTATGAATATCTCA
TTGAGCTTGCTGATGATGCAGAATTAGCAAAATTAGTGGAAGAACGCCAACACGAAAAAAGCTACAAAGTGGATTTAGCG
GATTTATTAAAATGA

Structures

Toxin

• AlphaFold DB: Q6DA89