Detailed information of TA system TA162012

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90089..90515
• Replicon: plasmid unnamed1
• Accession: NZ_CP055220
• Organism: Shigella flexneri strain STEFF_12

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HUZ41_RS24935
• Protein ID: WP_001372321.1
• Coordinates: 90089..90214 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	90291..90515 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ41_RS24895 (85459)	85459..86148	-	690	WP_000283379.1	conjugal transfer transcriptional regulator TraJ	-
HUZ41_RS24900 (86335)	86335..86718	-	384	WP_239637167.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HUZ41_RS24905 (87039)	87039..87641	+	603	WP_000243705.1	transglycosylase SLT domain-containing protein	-
HUZ41_RS24910 (87938)	87938..88759	-	822	WP_001241365.1	DUF932 domain-containing protein	-
HUZ41_RS24915 (88880)	88880..89167	-	288	WP_000107535.1	hypothetical protein	-
HUZ41_RS24920 (89192)	89192..89398	-	207	WP_033804333.1	hypothetical protein	-
HUZ41_RS24925 (89468)	89468..89641	+	174	Protein_92	hypothetical protein	-
HUZ41_RS24930 (89639)	89639..89869	-	231	WP_071845785.1	hypothetical protein	-
HUZ41_RS24935 (90089)	90089..90214	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUZ41_RS24940 (90156)	90156..90305	-	150	Protein_95	plasmid maintenance protein Mok	-
- (90293)	90293..90358	-	66	NuclAT_0	-	-
- (90293)	90293..90358	-	66	NuclAT_0	-	-
- (90293)	90293..90358	-	66	NuclAT_1	-	-
- (90296)	90296..90359	+	64	NuclAT_1	-	-
- (90296)	90296..90359	+	64	NuclAT_1	-	-
- (90291)	90291..90515	+	225	NuclAT_0	-	-
- (90291)	90291..90515	-	225	NuclAT_0	-	Antitoxin
- (90291)	90291..90515	-	225	NuclAT_0	-	Antitoxin
- (90291)	90291..90515	-	225	NuclAT_0	-	Antitoxin
- (90291)	90291..90515	-	225	NuclAT_0	-	Antitoxin
HUZ41_RS24945 (90327)	90327..90515	+	189	WP_001299721.1	hypothetical protein	-
HUZ41_RS24950 (90527)	90527..91246	-	720	WP_001276240.1	plasmid SOS inhibition protein A	-
HUZ41_RS24955 (91243)	91243..91677	-	435	WP_000845907.1	conjugation system SOS inhibitor PsiB	-
HUZ41_RS24960 (91732)	91732..93690	-	1959	WP_239637168.1	ParB/RepB/Spo0J family partition protein	-
HUZ41_RS24965 (93755)	93755..93988	-	234	WP_000006030.1	DUF905 family protein	-
HUZ41_RS24970 (94050)	94050..94502	-	453	WP_000290842.1	single-stranded DNA-binding protein	-
HUZ41_RS24975 (94528)	94528..94734	-	207	WP_000547968.1	hypothetical protein	-
HUZ41_RS24980 (94975)	94975..95208	-	234	WP_071588969.1	hypothetical protein	-
HUZ41_RS24985 (95145)	95145..95363	-	219	WP_071588970.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T162012 WP_001372321.1 NZ_CP055220:c90214-90089 [Shigella flexneri]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T162012 NZ_CP071464:c5059498-5058581 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT162012 NZ_CP055220:c90515-90291 [Shigella flexneri]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin