Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3850568..3851109 Replicon chromosome
Accession NZ_CP055219
Organism Shigella flexneri strain STEFF_12

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HUZ41_RS18840 Protein ID WP_000615976.1
Coordinates 3850568..3850846 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ41_RS18845 Protein ID WP_000729704.1
Coordinates 3850849..3851109 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ41_RS18825 (3848150) 3848150..3848728 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
HUZ41_RS18830 (3848934) 3848934..3849701 + 768 WP_000333379.1 class II glutamine amidotransferase -
HUZ41_RS18835 (3849672) 3849672..3850412 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ41_RS18840 (3850568) 3850568..3850846 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ41_RS18845 (3850849) 3850849..3851109 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ41_RS18850 (3851295) 3851295..3852068 + 774 WP_015675129.1 C40 family peptidase -
HUZ41_RS18855 (3852244) 3852244..3852741 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HUZ41_RS18860 (3852965) 3852965..3854677 - 1713 Protein_3689 flagellar biosynthesis protein FlhA -
HUZ41_RS18865 (3854649) 3854649..3855434 + 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T162009 WP_000615976.1 NZ_CP055219:c3850846-3850568 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T162009 NZ_CP071464:4185372-4185590 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT162009 WP_000729704.1 NZ_CP055219:c3851109-3850849 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT162009 NZ_CP071464:4184971-4185345 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References