Detailed information of TA system TA161987

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 239305..239676
• Replicon: chromosome
• Accession: NZ_CP055219
• Organism: Shigella flexneri strain STEFF_12

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: HUZ41_RS01275
• Protein ID: WP_001317028.1
• Coordinates: 239482..239676 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	239305..239483 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ41_RS25625 (235057)	235057..235230	+	174	WP_001296046.1	protein YnaL	-
HUZ41_RS01250 (235260)	235260..236633	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
HUZ41_RS01255 (236762)	236762..237697	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
HUZ41_RS01260 (237749)	237749..238984	-	1236	WP_000040852.1	site-specific integrase	-
HUZ41_RS01265 (238986)	238986..239201	-	216	WP_000079604.1	excisionase XisR	-
- (239305)	239305..239483	+	179	NuclAT_0	-	Antitoxin
- (239305)	239305..239483	+	179	NuclAT_0	-	Antitoxin
- (239305)	239305..239483	+	179	NuclAT_0	-	Antitoxin
- (239305)	239305..239483	+	179	NuclAT_0	-	Antitoxin
HUZ41_RS01270 (239280)	239280..239489	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
HUZ41_RS01275 (239482)	239482..239676	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HUZ41_RS01280 (239733)	239733..240542	-	810	WP_000166319.1	recombination protein RecT	-
HUZ41_RS01285 (240535)	240535..241884	-	1350	Protein_251	PD-(D/E)XK nuclease-like domain-containing protein	-
HUZ41_RS01295 (242646)	242646..243911	-	1266	Protein_253	exonuclease VIII	-
HUZ41_RS01300 (244013)	244013..244288	-	276	WP_000632297.1	protein RacC	-
HUZ41_RS01305 (244363)	244363..244533	-	171	WP_001352098.1	YdaE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	230490..249060	18570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T161987 WP_001317028.1 NZ_CP055219:c239676-239482 [Shigella flexneri]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T161987 NZ_CP071441:c4026360-4026082 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT161987 NZ_CP055219:239305-239483 [Shigella flexneri]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin