Detailed information of TA system TA161953

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62211..62480
• Replicon: plasmid unnamed2
• Accession: NZ_CP055212
• Organism: Klebsiella pneumoniae strain STEFF_2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HUZ38_RS27550
• Protein ID: WP_001372321.1
• Coordinates: 62355..62480 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	62211..62276 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ38_RS27520	57921..58448	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HUZ38_RS27525	58506..58739	+	234	WP_000006003.1	DUF905 family protein	-
HUZ38_RS27530	58800..60823	+	2024	Protein_72	ParB/RepB/Spo0J family partition protein	-
HUZ38_RS27535	60892..61326	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HUZ38_RS27540	61323..62042	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	62054..62278	+	225	NuclAT_0	-	-
-	62054..62278	+	225	NuclAT_0	-	-
-	62054..62278	+	225	NuclAT_0	-	-
-	62054..62278	+	225	NuclAT_0	-	-
-	62211..62276	+	66	-	-	Antitoxin
HUZ38_RS27545	62264..62413	+	150	Protein_75	plasmid maintenance protein Mok	-
HUZ38_RS27550	62355..62480	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUZ38_RS27555	62799..63095	-	297	Protein_77	hypothetical protein	-
HUZ38_RS27560	63395..63691	+	297	WP_001272251.1	hypothetical protein	-
HUZ38_RS27565	63802..64623	+	822	WP_001234445.1	DUF932 domain-containing protein	-
HUZ38_RS27570	64920..65567	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
HUZ38_RS27575	65844..66227	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HUZ38_RS27580	66418..67104	+	687	WP_015059009.1	PAS domain-containing protein	-
HUZ38_RS27585	67198..67425	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / sul2 / aph(3')-IIa / blaTEM-1B / rmtB / blaCTX-M-55	-	1..102776	102776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T161953 WP_001372321.1 NZ_CP055212:62355-62480 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T161953 NZ_CP071439:3406413-3406703 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT161953 NZ_CP055212:62211-62276 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin