Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4484715..4485312 Replicon chromosome
Accession NZ_CP055210
Organism Klebsiella pneumoniae strain STEFF_2

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HUZ38_RS21795 Protein ID WP_004142563.1
Coordinates 4484715..4485032 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HUZ38_RS21800 Protein ID WP_004142561.1
Coordinates 4485025..4485312 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ38_RS21780 (4480415) 4480415..4481842 - 1428 WP_009308097.1 MFS transporter -
HUZ38_RS21785 (4481951) 4481951..4482817 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
HUZ38_RS21790 (4483487) 4483487..4484530 + 1044 WP_020325241.1 DUF2157 domain-containing protein -
HUZ38_RS21795 (4484715) 4484715..4485032 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ38_RS21800 (4485025) 4485025..4485312 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HUZ38_RS21805 (4485577) 4485577..4486230 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
HUZ38_RS21810 (4486350) 4486350..4486718 - 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
HUZ38_RS21815 (4486718) 4486718..4487299 - 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
HUZ38_RS21820 (4487479) 4487479..4488594 + 1116 WP_020325248.1 MBL fold metallo-hydrolase -
HUZ38_RS21825 (4488625) 4488625..4488966 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HUZ38_RS21830 (4488984) 4488984..4489232 - 249 WP_020325243.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T161948 WP_004142563.1 NZ_CP055210:4484715-4485032 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T161948 NZ_CP071439:c2147098-2146784 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAATCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT161948 WP_004142561.1 NZ_CP055210:4485025-4485312 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT161948 NZ_CP071439:c2146787-2146371 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACGCCCAGGCGCTGGAACTGGTCGATCATCTGCTGCTCAACGATCCTGAAAATCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATTGGCAGTAAATCCATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References