Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 2653660..2654246 Replicon chromosome
Accession NZ_CP055210
Organism Klebsiella pneumoniae strain STEFF_2

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag HUZ38_RS13045 Protein ID WP_002920800.1
Coordinates 2653660..2654028 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag HUZ38_RS13050 Protein ID WP_004174006.1
Coordinates 2654025..2654246 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ38_RS13025 (2649163) 2649163..2650233 - 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
HUZ38_RS13030 (2650235) 2650235..2651080 - 846 WP_004181473.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
HUZ38_RS13035 (2651077) 2651077..2651964 - 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
HUZ38_RS13040 (2652071) 2652071..2653387 - 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
HUZ38_RS13045 (2653660) 2653660..2654028 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HUZ38_RS13050 (2654025) 2654025..2654246 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HUZ38_RS13055 (2654410) 2654410..2655123 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
HUZ38_RS13060 (2655125) 2655125..2655892 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HUZ38_RS13065 (2655889) 2655889..2657166 - 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
HUZ38_RS13070 (2657163) 2657163..2658089 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2648426..2669139 20713


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T161941 WP_002920800.1 NZ_CP055210:c2654028-2653660 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T161941 NZ_CP071439:c124103-123909 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT161941 WP_004174006.1 NZ_CP055210:c2654246-2654025 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT161941 NZ_CP071439:123732-123910 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References