Detailed information of TA system TA161918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4707171..4707773
• Replicon: chromosome
• Accession: NZ_CP055199
• Organism: Shigella flexneri strain STEFF_3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUZ39_RS23040
• Protein ID: WP_000897305.1
• Coordinates: 4707462..4707773 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ39_RS23035
• Protein ID: WP_000356397.1
• Coordinates: 4707171..4707461 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ39_RS23010 (4703116)	4703116..4704018	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HUZ39_RS23015 (4704015)	4704015..4704650	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUZ39_RS23020 (4704647)	4704647..4705576	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HUZ39_RS23025 (4705906)	4705906..4706148	-	243	WP_001087409.1	protein YiiF	-
HUZ39_RS23030 (4706367)	4706367..4706585	-	219	WP_001295676.1	CopG family transcriptional regulator	-
HUZ39_RS23035 (4707171)	4707171..4707461	-	291	WP_000356397.1	NadS family protein	Antitoxin
HUZ39_RS23040 (4707462)	4707462..4707773	-	312	WP_000897305.1	hypothetical protein	Toxin
HUZ39_RS23045 (4708002)	4708002..4708910	+	909	WP_001162704.1	alpha/beta hydrolase	-
HUZ39_RS23050 (4708974)	4708974..4709915	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HUZ39_RS23055 (4709960)	4709960..4710397	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HUZ39_RS23060 (4710394)	4710394..4711266	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HUZ39_RS23065 (4711260)	4711260..4711859	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T161918 WP_000897305.1 NZ_CP055199:c4707773-4707462 [Shigella flexneri]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T161918 NZ_CP071409:c2292196-2291891 [[Pantoea] beijingensis]
GTGACATCAGACGTCCAGTGGGAAATACGGGCACGGGCAGACCGAGAGGCTATCTTCCGCTATTTATATCAGGAAGCGGG
CCTGCCGGTCGCCAGCGCGACAGATGATAAGCTCGTCAGTATGGTGAGTATCCTCAGGGAAAACCCACTGGCAGGCGTTA
AAGCCGGGAGAACAGCAAAACAGCGTAAACTGGTTGTTCCCCACTTCCCGTTCATTATCGTCTATGTAGCGGAAGAAACA
GTGGTTCGTATCCTGCGGGTTCTGCATACATCCCGGAAAGTCGCAAGCCGTTACAGCAGAAGTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT161918 WP_000356397.1 NZ_CP055199:c4707461-4707171 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT161918 NZ_CP071409:c2292477-2292193 [[Pantoea] beijingensis]
ATGAGCACGATCCACTTCAGAATAGATGATGAAACAAAACGGCTGGCGATGCAGGCAGCAGAGCGCCAGAAGATGAGCCT
GACAGAACTCATGCGCCAGCGGGCTGAAGAACTGGCAGCAGAAGAACGACAGTATCAGGATGGTGAACATGATGCCTGGC
TGGAGCAGCAGATAGCCCTGGCGTTCAATCGCTATGATGCAGGTGAAAGCCAGTTCATCAGTAATGACGAAATGAACAGC
CATATGGATGAACTGAAAGCGCAGGCAGCGCGGGGCAAGCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7