Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3855824..3856365 Replicon chromosome
Accession NZ_CP055199
Organism Shigella flexneri strain STEFF_3

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag HUZ39_RS18930 Protein ID WP_000615982.1
Coordinates 3856087..3856365 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ39_RS18925 Protein ID WP_000729704.1
Coordinates 3855824..3856084 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ39_RS18900 (3850860) 3850860..3851999 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
HUZ39_RS18905 (3851983) 3851983..3854076 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
HUZ39_RS18910 (3854181) 3854181..3854459 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HUZ39_RS18915 (3854452) 3854452..3854808 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HUZ39_RS18920 (3854865) 3854865..3855638 - 774 WP_000543899.1 C40 family peptidase -
HUZ39_RS18925 (3855824) 3855824..3856084 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ39_RS18930 (3856087) 3856087..3856365 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ39_RS18935 (3856521) 3856521..3857261 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ39_RS18940 (3857232) 3857232..3857999 - 768 WP_000333380.1 class II glutamine amidotransferase -
HUZ39_RS18945 (3858205) 3858205..3858783 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T161915 WP_000615982.1 NZ_CP055199:3856087-3856365 [Shigella flexneri]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161915 NZ_CP071407:194575-194883 [Pantoea deleyi]
ATGCATGTAATATCACGTGTACCGTTTGACGAGGCGGTAAAAAAACATCCTAAGGCAGCGGCAGCACTGGAACTGCTTTA
TCGCAATCTCAGGCAGGGCGATTTTCGAACGCCTGAGGATCTCAGGGCGCTTTATGCAAGCCTCGATCGTATGAAATATC
GTGCCCGATGGTGGGTCATTGACCTGAGTGGCAATCAAATACGCGTCATGTTTTTTGCAGATTTTGAACGCAGTAAAATT
TTTATTAAACATATCTGCACACATGCTGAGTATGACAAGCTGGTGAAGAAATATCGGGAGGCAACCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT161915 WP_000729704.1 NZ_CP055199:3855824-3856084 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161915 NZ_CP071407:194883-195293 [Pantoea deleyi]
ATGAATAGTGAAGCTATCAGGCTGTCAAATCAGCTCGCGGAAGCATTACCCTTACTGGCGGGCAGCGGCCAGCAACGGGA
TTATGAAGATGCACTGGAACTGGTCGAATATCTCATTGAACATGCGCCGGATAATCCGCTGGTTGATCTGCTGTGTGCAC
GGATTGCGGATTATGAAGATAACGATCCCGGACTGGCCATATTTAATGCCCGTCAGAGGGAAATCGAAAGCGGCTCTGCT
GTGCTGCGTGTGCTGATTGAGCAGCACAACCTCCGGTTGAGTGACTTCGAAAACGAAATTGGCAAAAAATCACTGGTGAG
CAGAATCCTGAGCGGCGAGCGTCAGTTGACGGTGGCCCATATACGGGCGTTATGCGCCCGATTCGGCCTGTCGCCGTCGC
TGTTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References