Detailed information of TA system TA161879

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62032..62286
• Replicon: plasmid unnamed1
• Accession: NZ_CP055195
• Organism: Shigella flexneri strain STEFF_10

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUZ40_RS23860
• Protein ID: WP_001312851.1
• Coordinates: 62032..62181 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	62225..62286 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ40_RS23815 (57584)	57584..57985	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HUZ40_RS23820 (57918)	57918..58175	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HUZ40_RS23825 (58268)	58268..58921	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HUZ40_RS23830 (59019)	59019..59159	-	141	WP_001333237.1	hypothetical protein	-
HUZ40_RS23835 (59860)	59860..60717	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
HUZ40_RS23840 (60710)	60710..61192	-	483	WP_001273588.1	hypothetical protein	-
HUZ40_RS23845 (61185)	61185..61232	-	48	WP_229471593.1	hypothetical protein	-
HUZ40_RS23850 (61223)	61223..61474	+	252	WP_223195197.1	replication protein RepA	-
HUZ40_RS23855 (61491)	61491..61748	-	258	WP_000083845.1	replication regulatory protein RepA	-
HUZ40_RS23860 (62032)	62032..62181	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (62225)	62225..62286	+	62	NuclAT_1	-	Antitoxin
- (62225)	62225..62286	+	62	NuclAT_1	-	Antitoxin
- (62225)	62225..62286	+	62	NuclAT_1	-	Antitoxin
- (62225)	62225..62286	+	62	NuclAT_1	-	Antitoxin
HUZ40_RS23865 (62542)	62542..62616	-	75	Protein_69	endonuclease	-
HUZ40_RS23870 (62862)	62862..63074	-	213	WP_001299730.1	ANR family transcriptional regulator	-
HUZ40_RS23875 (63210)	63210..63770	-	561	WP_000139315.1	fertility inhibition protein FinO	-
HUZ40_RS23880 (63873)	63873..64733	-	861	WP_000704513.1	alpha/beta hydrolase	-
HUZ40_RS23885 (64792)	64792..65538	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / catA2 / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..125749	125749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T161879 WP_001312851.1 NZ_CP055195:c62181-62032 [Shigella flexneri]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T161879 NZ_CP071385:3960964-3961182 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT161879 NZ_CP055195:62225-62286 [Shigella flexneri]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin