Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4657108..4657710 Replicon chromosome
Accession NZ_CP055194
Organism Shigella flexneri strain STEFF_10

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag HUZ40_RS22530 Protein ID WP_000897305.1
Coordinates 4657399..4657710 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUZ40_RS22525 Protein ID WP_000356397.1
Coordinates 4657108..4657398 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ40_RS22500 (4653053) 4653053..4653955 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
HUZ40_RS22505 (4653952) 4653952..4654587 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
HUZ40_RS22510 (4654584) 4654584..4655513 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
HUZ40_RS22515 (4655843) 4655843..4656085 - 243 WP_001087409.1 protein YiiF -
HUZ40_RS22520 (4656304) 4656304..4656522 - 219 WP_001295676.1 CopG family transcriptional regulator -
HUZ40_RS22525 (4657108) 4657108..4657398 - 291 WP_000356397.1 NadS family protein Antitoxin
HUZ40_RS22530 (4657399) 4657399..4657710 - 312 WP_000897305.1 hypothetical protein Toxin
HUZ40_RS22535 (4657939) 4657939..4658847 + 909 WP_001162704.1 alpha/beta hydrolase -
HUZ40_RS22540 (4658911) 4658911..4659852 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
HUZ40_RS22545 (4659897) 4659897..4660334 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
HUZ40_RS22550 (4660331) 4660331..4661203 - 873 WP_000920762.1 virulence factor BrkB family protein -
HUZ40_RS22555 (4661197) 4661197..4661796 - 600 WP_001295269.1 glucose-1-phosphatase -
HUZ40_RS22560 (4661895) 4661895..4662680 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T161877 WP_000897305.1 NZ_CP055194:c4657710-4657399 [Shigella flexneri]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T161877 NZ_CP071385:1907013-1907115 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT161877 WP_000356397.1 NZ_CP055194:c4657398-4657108 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT161877 NZ_CP071385:c1907122-1906977 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References