Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3791640..3792181 Replicon chromosome
Accession NZ_CP055194
Organism Shigella flexneri strain STEFF_10

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag HUZ40_RS18485 Protein ID WP_000615982.1
Coordinates 3791903..3792181 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ40_RS18480 Protein ID WP_000729704.1
Coordinates 3791640..3791900 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ40_RS18455 (3786676) 3786676..3787815 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
HUZ40_RS18460 (3787799) 3787799..3789892 + 2094 WP_239637845.1 flagellar biosynthesis protein FlhA -
HUZ40_RS18465 (3789997) 3789997..3790275 + 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
HUZ40_RS18470 (3790268) 3790268..3790624 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HUZ40_RS18475 (3790681) 3790681..3791454 - 774 WP_000543895.1 C40 family peptidase -
HUZ40_RS18480 (3791640) 3791640..3791900 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ40_RS18485 (3791903) 3791903..3792181 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ40_RS18490 (3792337) 3792337..3793077 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ40_RS18495 (3793048) 3793048..3793815 - 768 WP_000333380.1 class II glutamine amidotransferase -
HUZ40_RS18500 (3794021) 3794021..3794599 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T161873 WP_000615982.1 NZ_CP055194:3791903-3792181 [Shigella flexneri]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161873 NZ_CP071385:324588-324956 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT161873 WP_000729704.1 NZ_CP055194:3791640-3791900 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161873 NZ_CP071385:324370-324591 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References