Detailed information of TA system TA161873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3791640..3792181
• Replicon: chromosome
• Accession: NZ_CP055194
• Organism: Shigella flexneri strain STEFF_10

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: E9TC49
• Locus tag: HUZ40_RS18485
• Protein ID: WP_000615982.1
• Coordinates: 3791903..3792181 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: S1EWQ0
• Locus tag: HUZ40_RS18480
• Protein ID: WP_000729704.1
• Coordinates: 3791640..3791900 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ40_RS18455 (3786676)	3786676..3787815	+	1140	WP_000785662.1	flagellar type III secretion system protein FlhB	-
HUZ40_RS18460 (3787799)	3787799..3789892	+	2094	WP_239637845.1	flagellar biosynthesis protein FlhA	-
HUZ40_RS18465 (3789997)	3789997..3790275	+	279	WP_000598758.1	type II toxin-antitoxin system HicA family toxin	-
HUZ40_RS18470 (3790268)	3790268..3790624	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
HUZ40_RS18475 (3790681)	3790681..3791454	-	774	WP_000543895.1	C40 family peptidase	-
HUZ40_RS18480 (3791640)	3791640..3791900	+	261	WP_000729704.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
HUZ40_RS18485 (3791903)	3791903..3792181	+	279	WP_000615982.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
HUZ40_RS18490 (3792337)	3792337..3793077	+	741	WP_001225679.1	peptidoglycan meso-diaminopimelic acid protein amidase	-
HUZ40_RS18495 (3793048)	3793048..3793815	-	768	WP_000333380.1	class II glutamine amidotransferase	-
HUZ40_RS18500 (3794021)	3794021..3794599	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10816.59 Da
• Isoelectric Point: 10.0702

>T161873 WP_000615982.1 NZ_CP055194:3791903-3792181 [Shigella flexneri]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T161873 NZ_CP071385:324588-324956 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9391.77 Da
• Isoelectric Point: 5.0758

>AT161873 WP_000729704.1 NZ_CP055194:3791640-3791900 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT161873 NZ_CP071385:324370-324591 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6Z6