Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3893251..3893848 Replicon chromosome
Accession NZ_CP055186
Organism Klebsiella pneumoniae strain STEFF_14

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HUZ42_RS19060 Protein ID WP_004142563.1
Coordinates 3893531..3893848 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HUZ42_RS19055 Protein ID WP_004142561.1
Coordinates 3893251..3893538 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ42_RS19025 (3889331) 3889331..3889579 + 249 WP_020325243.1 DUF1158 domain-containing protein -
HUZ42_RS19030 (3889597) 3889597..3889938 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HUZ42_RS19035 (3889969) 3889969..3891084 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
HUZ42_RS19040 (3891264) 3891264..3891845 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
HUZ42_RS19045 (3891845) 3891845..3892213 + 369 WP_239592435.1 MmcQ/YjbR family DNA-binding protein -
HUZ42_RS19050 (3892333) 3892333..3892986 + 654 WP_239592436.1 oxygen-insensitive NAD(P)H nitroreductase -
HUZ42_RS19055 (3893251) 3893251..3893538 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HUZ42_RS19060 (3893531) 3893531..3893848 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ42_RS19065 (3894033) 3894033..3895076 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
HUZ42_RS19070 (3895746) 3895746..3896612 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
HUZ42_RS19075 (3896721) 3896721..3898148 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T161847 WP_004142563.1 NZ_CP055186:c3893848-3893531 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T161847 NZ_CP071375:c3077602-3077270 [Escherichia coli]
ATGGACAGAGGAGAAATCTGGCTCGTTTCACTGGATCCCACCACTGGCCATGAACAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCGTTTAACACGTTCACCCGGTTGCCTGTTGTTGTCCCTGTCACCAGCGGCGGAAACTTTGCCCGCG
AAGCCGGTTTTACCGTTTCACTGGACGGGGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCTCGGAGCGGTAAGCGGCTGGAACGTATACCCGATGCTGTCGTGAATGAGGTGCTGGCTCGACTGGAAGC
TATTCTGAGCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT161847 WP_004142561.1 NZ_CP055186:c3893538-3893251 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT161847 NZ_CP071375:c3077860-3077603 [Escherichia coli]
ATGTATACCACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCCCCCGCCGTGCTGAAAACGCTAGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATGATGGCTGCCTGATTATTGAACCTCAAAAACGTCCCCGCTATTCGC
TCGAAGAACTGTTGGCGCAGTGCGATCCGCACGCTGAAATAAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References