Detailed information of TA system TA161833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4552931..4553533
• Replicon: chromosome
• Accession: NZ_CP055184
• Organism: Shigella flexneri strain STEFF_16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUZ44_RS21800
• Protein ID: WP_000897305.1
• Coordinates: 4553222..4553533 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ44_RS21795
• Protein ID: WP_000356397.1
• Coordinates: 4552931..4553221 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ44_RS21770 (4548856)	4548856..4549758	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HUZ44_RS21775 (4549755)	4549755..4550390	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUZ44_RS21780 (4550387)	4550387..4551316	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HUZ44_RS21785 (4551646)	4551646..4551888	-	243	WP_001087409.1	protein YiiF	-
HUZ44_RS21790 (4552108)	4552108..4552326	-	219	WP_001315930.1	CopG family transcriptional regulator	-
HUZ44_RS21795 (4552931)	4552931..4553221	-	291	WP_000356397.1	NadS family protein	Antitoxin
HUZ44_RS21800 (4553222)	4553222..4553533	-	312	WP_000897305.1	hypothetical protein	Toxin
HUZ44_RS21805 (4553762)	4553762..4554670	+	909	WP_001162704.1	alpha/beta hydrolase	-
HUZ44_RS21810 (4554734)	4554734..4555675	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HUZ44_RS21815 (4555720)	4555720..4556157	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HUZ44_RS21820 (4556154)	4556154..4557026	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HUZ44_RS21825 (4557020)	4557020..4557619	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T161833 WP_000897305.1 NZ_CP055184:c4553533-4553222 [Shigella flexneri]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T161833 NZ_CP071324:c4214431-4214213 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT161833 WP_000356397.1 NZ_CP055184:c4553221-4552931 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT161833 NZ_CP071324:c4214832-4214458 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7