Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3720826..3721367 Replicon chromosome
Accession NZ_CP055184
Organism Shigella flexneri strain STEFF_16

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag HUZ44_RS17985 Protein ID WP_000615982.1
Coordinates 3721089..3721367 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ44_RS17980 Protein ID WP_000729704.1
Coordinates 3720826..3721086 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ44_RS17955 (3716609) 3716609..3717394 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
HUZ44_RS17960 (3717366) 3717366..3719078 + 1713 Protein_3527 flagellar biosynthesis protein FlhA -
HUZ44_RS17965 (3719183) 3719183..3719461 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HUZ44_RS17970 (3719454) 3719454..3719810 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HUZ44_RS17975 (3719867) 3719867..3720640 - 774 WP_000543899.1 C40 family peptidase -
HUZ44_RS17980 (3720826) 3720826..3721086 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ44_RS17985 (3721089) 3721089..3721367 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ44_RS17990 (3721523) 3721523..3722263 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ44_RS17995 (3722234) 3722234..3723001 - 768 WP_000333380.1 class II glutamine amidotransferase -
HUZ44_RS18000 (3723207) 3723207..3723785 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T161830 WP_000615982.1 NZ_CP055184:3721089-3721367 [Shigella flexneri]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161830 NZ_CP071324:2995479-2995862 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCAGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT161830 WP_000729704.1 NZ_CP055184:3720826-3721086 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161830 NZ_CP071324:2995237-2995479 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References