Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3774235..3774776 Replicon chromosome
Accession NZ_CP055179
Organism Shigella flexneri strain STEFF_18

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HUZ46_RS18315 Protein ID WP_000615976.1
Coordinates 3774498..3774776 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ46_RS18310 Protein ID WP_000729704.1
Coordinates 3774235..3774495 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ46_RS18285 (3770018) 3770018..3770803 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
HUZ46_RS18290 (3770775) 3770775..3772487 + 1713 Protein_3591 flagellar biosynthesis protein FlhA -
HUZ46_RS18295 (3772592) 3772592..3772870 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HUZ46_RS18300 (3772863) 3772863..3773219 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HUZ46_RS18305 (3773276) 3773276..3774049 - 774 WP_000543896.1 C40 family peptidase -
HUZ46_RS18310 (3774235) 3774235..3774495 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ46_RS18315 (3774498) 3774498..3774776 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ46_RS18320 (3774932) 3774932..3775672 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ46_RS18325 (3775643) 3775643..3776410 - 768 WP_000333380.1 class II glutamine amidotransferase -
HUZ46_RS18330 (3776616) 3776616..3777194 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T161778 WP_000615976.1 NZ_CP055179:3774498-3774776 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161778 NZ_CP071279:480813-481223 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGAAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT161778 WP_000729704.1 NZ_CP055179:3774235-3774495 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161778 NZ_CP071279:480566-480832 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References