Detailed information of TA system TA161765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1086963..1087690
• Replicon: chromosome
• Accession: NZ_CP055179
• Organism: Shigella flexneri strain STEFF_18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: HUZ46_RS05225
• Protein ID: WP_000547563.1
• Coordinates: 1086963..1087274 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ46_RS05230
• Protein ID: WP_000126294.1
• Coordinates: 1087271..1087690 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ46_RS05195 (1082106)	1082106..1083815	+	1710	WP_063625439.1	formate hydrogenlyase subunit HycE	-
HUZ46_RS05200 (1083825)	1083825..1084367	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
HUZ46_RS05205 (1084367)	1084367..1085134	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
HUZ46_RS05210 (1085131)	1085131..1085541	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
HUZ46_RS05215 (1085534)	1085534..1086004	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HUZ46_RS05220 (1086047)	1086047..1086790	+	744	WP_058101263.1	hypothetical protein	-
HUZ46_RS05225 (1086963)	1086963..1087274	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
HUZ46_RS05230 (1087271)	1087271..1087690	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
HUZ46_RS05235 (1087804)	1087804..1089228	-	1425	WP_000110365.1	6-phospho-beta-glucosidase AscB	-
HUZ46_RS05240 (1089237)	1089237..1090694	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HUZ46_RS05245 (1090954)	1090954..1091964	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
HUZ46_RS05250 (1092113)	1092113..1092640	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T161765 WP_000547563.1 NZ_CP055179:1086963-1087274 [Shigella flexneri]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T161765 NZ_CP071265:c356997-356680 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAAGGATTTAGTAAACGCCGTCCGTTACTTTTGCCTGACGATGAGTTTCGCGCTTTTCA
AGAGGCGCTTATAGAGAACCCTGAGGCTGGAGATACTATCGCTGGTACAGGTGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGCAAACGCAGCGGTATTAGGGTTATCTATTACAACGTGACGCGCAAAGGTCGAATCTATCTCGCTTTGCTC
TATCCTAAAAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCAATATGCTGATTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT161765 WP_000126294.1 NZ_CP055179:1087271-1087690 [Shigella flexneri]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT161765 NZ_CP071265:c356627-356331 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTGCGTATAGAGAAAGGTGAAGAAACACCTAA
GCCTGAACATGTACATACCTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCTGTTGGCGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAGCCGACTGGTGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAATCCATTTATAATTAATCAATTAAAAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22