Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 641210..642009 Replicon chromosome
Accession NZ_CP055179
Organism Shigella flexneri strain STEFF_18

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag HUZ46_RS03120 Protein ID WP_000347267.1
Coordinates 641210..641674 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HUZ46_RS03125 Protein ID WP_001307405.1
Coordinates 641674..642009 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ46_RS03090 (636211) 636211..636645 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HUZ46_RS03095 (636663) 636663..637541 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
HUZ46_RS03100 (637531) 637531..638310 - 780 WP_063625504.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUZ46_RS03105 (638321) 638321..638794 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUZ46_RS03110 (638817) 638817..640097 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUZ46_RS03115 (640346) 640346..641155 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUZ46_RS03120 (641210) 641210..641674 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUZ46_RS03125 (641674) 641674..642009 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUZ46_RS03130 (642158) 642158..643729 - 1572 WP_001273741.1 galactarate dehydratase -
HUZ46_RS03135 (644104) 644104..645438 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HUZ46_RS03140 (645454) 645454..646224 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 641210..654152 12942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T161762 WP_000347267.1 NZ_CP055179:c641674-641210 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T161762 NZ_CP071263:c5175584-5175477 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT161762 WP_001307405.1 NZ_CP055179:c642009-641674 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT161762 NZ_CP071263:5175637-5175698 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References