Detailed information of TA system TA161757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 69836..70563
• Replicon: plasmid unnamed1
• Accession: NZ_CP055175
• Organism: Klebsiella pneumoniae strain STEFF_19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HUZ47_RS26350
• Protein ID: WP_011251285.1
• Coordinates: 69836..70147 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ47_RS26355
• Protein ID: WP_011251286.1
• Coordinates: 70144..70563 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ47_RS26320 (HUZ47_26285)	65027..66007	+	981	WP_012569499.1	IS5-like element ISKpn26 family transposase	-
HUZ47_RS26325 (HUZ47_26290)	66553..66732	-	180	Protein_71	IS5/IS1182 family transposase	-
HUZ47_RS26330 (HUZ47_26295)	66772..68310	-	1539	WP_017901237.1	IS66 family transposase	-
HUZ47_RS26335 (HUZ47_26300)	68359..68706	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
HUZ47_RS26340 (HUZ47_26305)	68703..69080	-	378	WP_004118218.1	transposase	-
HUZ47_RS26345 (HUZ47_26310)	69194..69631	+	438	Protein_75	DDE-type integrase/transposase/recombinase	-
HUZ47_RS26350 (HUZ47_26315)	69836..70147	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HUZ47_RS26355 (HUZ47_26320)	70144..70563	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HUZ47_RS26360 (HUZ47_26325)	70633..71046	+	414	WP_017901337.1	hypothetical protein	-
HUZ47_RS26365 (HUZ47_26330)	71092..72060	-	969	WP_074168518.1	IS5-like element IS903B family transposase	-
HUZ47_RS26370 (HUZ47_26335)	72164..72430	+	267	WP_017900868.1	hypothetical protein	-
HUZ47_RS26375 (HUZ47_26340)	72544..73079	-	536	Protein_81	transposase	-
HUZ47_RS26380 (HUZ47_26345)	73604..74971	-	1368	WP_017900870.1	formimidoylglutamate deiminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / tet(D) / aph(6)-Id / aph(3'')-Ib / catA1 / sul2	-	1..233421	233421
	-	inside	IScluster/Tn	-	-	65027..72762	7735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T161757 WP_011251285.1 NZ_CP055175:69836-70147 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T161757 NZ_CP071263:3559537-3559887 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT161757 WP_011251286.1 NZ_CP055175:70144-70563 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT161757 NZ_CP071263:3559292-3559543 [Escherichia coli]
ATGCGTATTACCATAAAAAAATGGGGGAACAGTTCAGGGATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1