Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 69836..70563 Replicon plasmid unnamed1
Accession NZ_CP055175
Organism Klebsiella pneumoniae strain STEFF_19

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag HUZ47_RS26350 Protein ID WP_011251285.1
Coordinates 69836..70147 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUZ47_RS26355 Protein ID WP_011251286.1
Coordinates 70144..70563 (+) Length 140 a.a.

Genomic Context

HUZ47_RS26320HUZ47_RS26345HUZ47_RS26350HUZ47_RS26355HUZ47_RS26360HUZ47_RS26370HUZ47_RS26325HUZ47_RS26330HUZ47_RS26335HUZ47_RS26340HUZ47_RS26365HUZ47_RS26375HUZ47_RS26380
Location: 65027..66007 (981 bp)
Type: Others
Protein ID: WP_012569499.1
Location: 69194..69631 (438 bp)
Type: Others
Protein ID: Protein_75
Location: 69836..70147 (312 bp)
Type: Toxin
Protein ID: WP_011251285.1
Location: 70144..70563 (420 bp)
Type: Antitoxin
Protein ID: WP_011251286.1
Location: 70633..71046 (414 bp)
Type: Others
Protein ID: WP_017901337.1
Location: 72164..72430 (267 bp)
Type: Others
Protein ID: WP_017900868.1
Location: 66553..66732 (180 bp)
Type: Others
Protein ID: Protein_71
Location: 66772..68310 (1539 bp)
Type: Others
Protein ID: WP_017901237.1
Location: 68359..68706 (348 bp)
Type: Others
Protein ID: WP_032430752.1
Location: 68703..69080 (378 bp)
Type: Others
Protein ID: WP_004118218.1
Location: 71092..72060 (969 bp)
Type: Others
Protein ID: WP_074168518.1
Location: 72544..73079 (536 bp)
Type: Others
Protein ID: Protein_81
Location: 73604..74971 (1368 bp)
Type: Others
Protein ID: WP_017900870.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
HUZ47_RS26320 (HUZ47_26285)65027..66007+981WP_012569499.1IS5-like element ISKpn26 family transposase-
HUZ47_RS26325 (HUZ47_26290)66553..66732-180Protein_71IS5/IS1182 family transposase-
HUZ47_RS26330 (HUZ47_26295)66772..68310-1539WP_017901237.1IS66 family transposase-
HUZ47_RS26335 (HUZ47_26300)68359..68706-348WP_032430752.1IS66 family insertion sequence element accessory protein TnpB-
HUZ47_RS26340 (HUZ47_26305)68703..69080-378WP_004118218.1transposase-
HUZ47_RS26345 (HUZ47_26310)69194..69631+438Protein_75DDE-type integrase/transposase/recombinase-
HUZ47_RS26350 (HUZ47_26315)69836..70147+312WP_011251285.1type II toxin-antitoxin system HigB family toxinToxin
HUZ47_RS26355 (HUZ47_26320)70144..70563+420WP_011251286.1helix-turn-helix domain-containing proteinAntitoxin
HUZ47_RS26360 (HUZ47_26325)70633..71046+414WP_017901337.1hypothetical protein-
HUZ47_RS26365 (HUZ47_26330)71092..72060-969WP_074168518.1IS5-like element IS903B family transposase-
HUZ47_RS26370 (HUZ47_26335)72164..72430+267WP_017900868.1hypothetical protein-
HUZ47_RS26375 (HUZ47_26340)72544..73079-536Protein_81transposase-
HUZ47_RS26380 (HUZ47_26345)73604..74971-1368WP_017900870.1formimidoylglutamate deiminase-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
-insideNon-Mobilizable plasmidaph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / tet(D) / aph(6)-Id / aph(3'')-Ib / catA1 / sul2-1..233421233421
-insideIScluster/Tn--65027..727627735


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T161757 WP_011251285.1 NZ_CP055175:69836-70147 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T161757 NZ_CP071263:3559537-3559887 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT161757 WP_011251286.1 NZ_CP055175:70144-70563 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT161757 NZ_CP071263:3559292-3559543 [Escherichia coli]
ATGCGTATTACCATAAAAAAATGGGGGAACAGTTCAGGGATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found
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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found

Structures

Toxin

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Source
ID
Structure
AlphaFold DBA0A7W3D9W1

Antitoxin

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Source
ID
Structure
No matching records found

References