Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 332854..333440 Replicon chromosome
Accession NZ_CP055174
Organism Klebsiella pneumoniae strain STEFF_19

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag HUZ47_RS01550 Protein ID WP_002920800.1
Coordinates 333072..333440 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag HUZ47_RS01545 Protein ID WP_004174006.1
Coordinates 332854..333075 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ47_RS01525 (HUZ47_01520) 329011..329937 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HUZ47_RS01530 (HUZ47_01525) 329934..331211 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
HUZ47_RS01535 (HUZ47_01530) 331208..331975 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HUZ47_RS01540 (HUZ47_01535) 331977..332690 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
HUZ47_RS01545 (HUZ47_01540) 332854..333075 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HUZ47_RS01550 (HUZ47_01545) 333072..333440 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HUZ47_RS01555 (HUZ47_01550) 333713..335029 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
HUZ47_RS01560 (HUZ47_01555) 335136..336023 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
HUZ47_RS01565 (HUZ47_01560) 336020..336865 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
HUZ47_RS01570 (HUZ47_01565) 336867..337937 + 1071 WP_017880090.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 327840..338674 10834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T161737 WP_002920800.1 NZ_CP055174:333072-333440 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T161737 NZ_CP071261:c4691694-4691410 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT161737 WP_004174006.1 NZ_CP055174:332854-333075 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT161737 NZ_CP071261:c4691926-4691684 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGATTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References