Detailed information of TA system TA161733

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 91223..91649
• Replicon: plasmid unnamed1
• Accession: NZ_CP055171
• Organism: Shigella flexneri strain STEFF_22

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HUZ49_RS23670
• Protein ID: WP_001372321.1
• Coordinates: 91223..91348 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	91425..91649 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ49_RS23630 (86373)	86373..86774	-	402	WP_001369361.1	conjugal transfer relaxosome DNA-bindin protein TraY	-
HUZ49_RS23635 (86867)	86867..87556	-	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-
HUZ49_RS23640 (87743)	87743..88126	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HUZ49_RS23645 (88447)	88447..89049	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HUZ49_RS23650 (89346)	89346..90167	-	822	WP_072795862.1	DUF932 domain-containing protein	-
HUZ49_RS23655 (90289)	90289..90576	-	288	WP_000107535.1	hypothetical protein	-
HUZ49_RS23660 (90578)	90578..90775	+	198	Protein_102	hypothetical protein	-
HUZ49_RS23665 (90773)	90773..91003	-	231	WP_001426396.1	hypothetical protein	-
HUZ49_RS23670 (91223)	91223..91348	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUZ49_RS23675 (91290)	91290..91439	-	150	Protein_105	plasmid maintenance protein Mok	-
- (91425)	91425..91649	-	225	NuclAT_0	-	Antitoxin
- (91425)	91425..91649	-	225	NuclAT_0	-	Antitoxin
- (91425)	91425..91649	-	225	NuclAT_0	-	Antitoxin
- (91425)	91425..91649	-	225	NuclAT_0	-	Antitoxin
HUZ49_RS23680 (91461)	91461..91649	+	189	WP_001299721.1	hypothetical protein	-
HUZ49_RS23685 (91661)	91661..92380	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
HUZ49_RS23690 (92377)	92377..92811	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HUZ49_RS23695 (92866)	92866..94824	-	1959	WP_039000998.1	ParB/RepB/Spo0J family partition protein	-
HUZ49_RS23700 (94889)	94889..95122	-	234	WP_000005987.1	DUF905 family protein	-
HUZ49_RS23705 (95179)	95179..95718	-	540	WP_032332913.1	single-stranded DNA-binding protein	-
HUZ49_RS23710 (95744)	95744..95950	-	207	WP_000275853.1	hypothetical protein	-
HUZ49_RS23715 (96191)	96191..96418	-	228	WP_071961421.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / aph(3')-Ia / sul3 / blaTEM-1B / lnu(F) / aac(3)-IId	-	1..131772	131772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T161733 WP_001372321.1 NZ_CP055171:c91348-91223 [Shigella flexneri]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T161733 NZ_CP071261:c2625845-2624523 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTTACCCTCCTGCCGCCGGG
GGAAGAAGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGCGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCTGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTGCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTCACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT161733 NZ_CP055171:c91649-91425 [Shigella flexneri]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin