Detailed information of TA system TA161729

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 56199..56453
• Replicon: plasmid unnamed1
• Accession: NZ_CP055171
• Organism: Shigella flexneri strain STEFF_22

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUZ49_RS23480
• Protein ID: WP_001312851.1
• Coordinates: 56199..56348 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	56392..56453 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ49_RS23445 (52220)	52220..52720	+	501	Protein_59	Tn3 family transposase	-
HUZ49_RS23450 (52754)	52754..52942	+	189	Protein_60	IS3 family transposase	-
HUZ49_RS23455 (53017)	53017..53304	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HUZ49_RS23460 (53301)	53301..53552	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HUZ49_RS23465 (54518)	54518..55375	-	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
HUZ49_RS23470 (55368)	55368..55442	-	75	WP_061357229.1	RepA leader peptide Tap	-
HUZ49_RS23475 (55674)	55674..55931	-	258	WP_001541895.1	replication regulatory protein RepA	-
HUZ49_RS23480 (56199)	56199..56348	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (56392)	56392..56453	+	62	NuclAT_1	-	Antitoxin
- (56392)	56392..56453	+	62	NuclAT_1	-	Antitoxin
- (56392)	56392..56453	+	62	NuclAT_1	-	Antitoxin
- (56392)	56392..56453	+	62	NuclAT_1	-	Antitoxin
HUZ49_RS23485 (56795)	56795..57007	-	213	WP_001541896.1	ANR family transcriptional regulator	-
HUZ49_RS23490 (57142)	57142..57702	-	561	WP_000139310.1	fertility inhibition protein FinO	-
HUZ49_RS23495 (57757)	57757..58500	-	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / aph(3')-Ia / sul3 / blaTEM-1B / lnu(F) / aac(3)-IId	-	1..131772	131772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T161729 WP_001312851.1 NZ_CP055171:c56348-56199 [Shigella flexneri]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T161729 NZ_CP071261:322422-322790 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT161729 NZ_CP055171:56392-56453 [Shigella flexneri]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin