Detailed information of TA system TA161727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4785058..4785660
• Replicon: chromosome
• Accession: NZ_CP055170
• Organism: Shigella flexneri strain STEFF_22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUZ49_RS22975
• Protein ID: WP_000897305.1
• Coordinates: 4785058..4785369 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ49_RS22980
• Protein ID: WP_000356397.1
• Coordinates: 4785370..4785660 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ49_RS22945 (4780088)	4780088..4780873	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
HUZ49_RS22950 (4780972)	4780972..4781571	+	600	WP_001295269.1	glucose-1-phosphatase	-
HUZ49_RS22955 (4781565)	4781565..4782437	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HUZ49_RS22960 (4782434)	4782434..4782871	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HUZ49_RS22965 (4782916)	4782916..4783857	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
HUZ49_RS22970 (4783921)	4783921..4784829	-	909	WP_001162704.1	alpha/beta hydrolase	-
HUZ49_RS22975 (4785058)	4785058..4785369	+	312	WP_000897305.1	hypothetical protein	Toxin
HUZ49_RS22980 (4785370)	4785370..4785660	+	291	WP_000356397.1	NadS family protein	Antitoxin
HUZ49_RS22985 (4786265)	4786265..4786483	+	219	WP_001297075.1	CopG family transcriptional regulator	-
HUZ49_RS22990 (4786702)	4786702..4786944	+	243	WP_001086388.1	protein YiiF	-
HUZ49_RS22995 (4787274)	4787274..4788203	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HUZ49_RS23000 (4788200)	4788200..4788835	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUZ49_RS23005 (4788832)	4788832..4789734	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T161727 WP_000897305.1 NZ_CP055170:4785058-4785369 [Shigella flexneri]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T161727 NZ_CP071259:c4682284-4682111 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT161727 WP_000356397.1 NZ_CP055170:4785370-4785660 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT161727 NZ_CP071259:c4682608-4682312 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7