Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3957209..3958008 Replicon chromosome
Accession NZ_CP055170
Organism Shigella flexneri strain STEFF_22

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag HUZ49_RS18975 Protein ID WP_000347267.1
Coordinates 3957544..3958008 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HUZ49_RS18970 Protein ID WP_001307405.1
Coordinates 3957209..3957544 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ49_RS18955 (3952994) 3952994..3953764 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
HUZ49_RS18960 (3953780) 3953780..3955114 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HUZ49_RS18965 (3955489) 3955489..3957060 + 1572 WP_001273741.1 galactarate dehydratase -
HUZ49_RS18970 (3957209) 3957209..3957544 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUZ49_RS18975 (3957544) 3957544..3958008 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUZ49_RS18980 (3958063) 3958063..3958872 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUZ49_RS18985 (3959121) 3959121..3960401 + 1281 WP_000681910.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUZ49_RS18990 (3960424) 3960424..3960897 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUZ49_RS18995 (3960908) 3960908..3961687 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUZ49_RS19000 (3961677) 3961677..3962555 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HUZ49_RS19005 (3962573) 3962573..3963007 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3946334..3958008 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T161725 WP_000347267.1 NZ_CP055170:3957544-3958008 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T161725 NZ_CP071259:c4334209-4333922 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT161725 WP_001307405.1 NZ_CP055170:3957209-3957544 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT161725 NZ_CP071259:c4333935-4333633 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCAATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References