Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2634785..2635287 Replicon chromosome
Accession NZ_CP055170
Organism Shigella flexneri strain STEFF_22

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HUZ49_RS12735 Protein ID WP_000767829.1
Coordinates 2634785..2635039 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HUZ49_RS12740 Protein ID WP_001259255.1
Coordinates 2635036..2635287 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ49_RS12705 (2629800) 2629800..2630027 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
HUZ49_RS12710 (2630041) 2630041..2631099 - 1059 WP_000492346.1 thiosulfate utilization transporter TsuA/YeeE -
HUZ49_RS12715 (2631278) 2631278..2632636 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HUZ49_RS12720 (2632626) 2632626..2632688 - 63 WP_010723108.1 membrane protein YoeI -
HUZ49_RS12725 (2632903) 2632903..2633832 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
HUZ49_RS12730 (2633878) 2633878..2634702 - 825 WP_000754749.1 SDR family oxidoreductase -
HUZ49_RS12735 (2634785) 2634785..2635039 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HUZ49_RS12740 (2635036) 2635036..2635287 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HUZ49_RS12745 (2635570) 2635570..2635620 + 51 WP_001364200.1 his operon leader peptide -
HUZ49_RS12750 (2635766) 2635766..2636665 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HUZ49_RS12755 (2636671) 2636671..2637975 + 1305 WP_063108230.1 histidinol dehydrogenase -
HUZ49_RS12760 (2637972) 2637972..2639042 + 1071 WP_000108970.1 histidinol-phosphate transaminase -
HUZ49_RS12765 (2639042) 2639042..2640109 + 1068 WP_063108231.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T161720 WP_000767829.1 NZ_CP055170:c2635039-2634785 [Shigella flexneri]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T161720 NZ_CP071259:2839751-2839858 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT161720 WP_001259255.1 NZ_CP055170:c2635287-2635036 [Shigella flexneri]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT161720 NZ_CP071259:c2839698-2839637 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References