Detailed information of TA system TA161695

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60058..60312
• Replicon: plasmid unnamed1
• Accession: NZ_CP055168
• Organism: Shigella flexneri strain STEFF_24

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUZ50_RS23885
• Protein ID: WP_001312851.1
• Coordinates: 60058..60207 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	60251..60312 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ50_RS23845 (55610)	55610..56011	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HUZ50_RS23850 (55944)	55944..56201	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HUZ50_RS23855 (56294)	56294..56947	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HUZ50_RS23860 (57886)	57886..58743	-	858	WP_239612969.1	incFII family plasmid replication initiator RepA	-
HUZ50_RS23865 (58736)	58736..59218	-	483	WP_001273588.1	hypothetical protein	-
HUZ50_RS23870 (59211)	59211..59258	-	48	WP_229471593.1	hypothetical protein	-
HUZ50_RS23875 (59249)	59249..59500	+	252	WP_223195197.1	replication protein RepA	-
HUZ50_RS23880 (59517)	59517..59774	-	258	WP_000083845.1	replication regulatory protein RepA	-
HUZ50_RS23885 (60058)	60058..60207	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (60251)	60251..60312	+	62	NuclAT_1	-	Antitoxin
- (60251)	60251..60312	+	62	NuclAT_1	-	Antitoxin
- (60251)	60251..60312	+	62	NuclAT_1	-	Antitoxin
- (60251)	60251..60312	+	62	NuclAT_1	-	Antitoxin
HUZ50_RS23890 (60568)	60568..60642	-	75	Protein_70	endonuclease	-
HUZ50_RS23895 (60888)	60888..61100	-	213	WP_001299730.1	ANR family transcriptional regulator	-
HUZ50_RS23900 (61236)	61236..61796	-	561	WP_000139315.1	fertility inhibition protein FinO	-
HUZ50_RS23905 (61899)	61899..62759	-	861	WP_000704513.1	alpha/beta hydrolase	-
HUZ50_RS23910 (62818)	62818..63564	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaCTX-M-15 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..125110	125110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T161695 WP_001312851.1 NZ_CP055168:c60207-60058 [Shigella flexneri]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T161695 NZ_CP071252:325513-325881 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT161695 NZ_CP055168:60251-60312 [Shigella flexneri]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin