Detailed information of TA system TA161693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4703190..4703792
• Replicon: chromosome
• Accession: NZ_CP055167
• Organism: Shigella flexneri strain STEFF_24

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUZ50_RS22565
• Protein ID: WP_000897305.1
• Coordinates: 4703481..4703792 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ50_RS22560
• Protein ID: WP_000356397.1
• Coordinates: 4703190..4703480 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ50_RS22535 (4698622)	4698622..4699257	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUZ50_RS22540 (4699254)	4699254..4700183	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HUZ50_RS22545 (4700696)	4700696..4701676	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
HUZ50_RS22550 (4701906)	4701906..4702148	-	243	WP_001086388.1	protein YiiF	-
HUZ50_RS22555 (4702367)	4702367..4702585	-	219	WP_001295676.1	CopG family transcriptional regulator	-
HUZ50_RS22560 (4703190)	4703190..4703480	-	291	WP_000356397.1	NadS family protein	Antitoxin
HUZ50_RS22565 (4703481)	4703481..4703792	-	312	WP_000897305.1	hypothetical protein	Toxin
HUZ50_RS22570 (4704021)	4704021..4704929	+	909	WP_001162704.1	alpha/beta hydrolase	-
HUZ50_RS22575 (4704993)	4704993..4705934	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HUZ50_RS22580 (4705979)	4705979..4706416	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HUZ50_RS22585 (4706413)	4706413..4707285	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HUZ50_RS22590 (4707279)	4707279..4707878	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4700696..4701676	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T161693 WP_000897305.1 NZ_CP055167:c4703792-4703481 [Shigella flexneri]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T161693 NZ_CP071248:2233264-2233518 [Bifidobacterium longum subsp. infantis]
ATGCTGCTCTGCTGGACCGAAGACGCCTGGGCCGACTACCTGTACTGGCAATCCCAGGACCGCAAGACCCTCAAACGCGT
CAACACGCTCATCCGGGACATGCAGCGAACCCCGTTCGAAGGCATCGGCAAGCCCGAACCCCTCAAATGGGGCCTGTCCG
GCGCATGGTCGCGGCGCATCGATTCGGCCAACCGCATCATCTACACCGTCGCCGACGACAGGCTCTGCACCCTCTCCGCG
AAGGACCACTACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT161693 WP_000356397.1 NZ_CP055167:c4703480-4703190 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT161693 NZ_CP071248:2232998-2233264 [Bifidobacterium longum subsp. infantis]
ATGACACAGGCCGTGGCCTACAGCAATTTCAGGGCGAATCTCAAGACCTACATGCGCAGGGTCAACGAGGACGCCGATAC
CCTTCTCGTGACCAACGCGGACCCGGAGGACAACGTGGTCGTGATGAGCGCCGACGACTACGATTCGCTCATGGAGACGC
TGCGCGTCTACCAGAACCCGTATCTGAGCGATAAGGTGATGCGCGGCATGGCGCAGGTCCGGCAGGGCCAGACGATGGCC
CACGACCTTGTCGAAGCGGGCGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7