Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3774234..3774775 Replicon chromosome
Accession NZ_CP055167
Organism Shigella flexneri strain STEFF_24

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HUZ50_RS18315 Protein ID WP_000615976.1
Coordinates 3774497..3774775 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUZ50_RS18310 Protein ID WP_000729704.1
Coordinates 3774234..3774494 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ50_RS18285 (3770017) 3770017..3770802 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
HUZ50_RS18290 (3770774) 3770774..3772486 + 1713 Protein_3591 flagellar biosynthesis protein FlhA -
HUZ50_RS18295 (3772591) 3772591..3772869 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HUZ50_RS18300 (3772862) 3772862..3773218 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HUZ50_RS18305 (3773275) 3773275..3774048 - 774 WP_000543896.1 C40 family peptidase -
HUZ50_RS18310 (3774234) 3774234..3774494 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUZ50_RS18315 (3774497) 3774497..3774775 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUZ50_RS18320 (3774931) 3774931..3775671 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUZ50_RS18325 (3775642) 3775642..3776409 - 768 WP_000333380.1 class II glutamine amidotransferase -
HUZ50_RS18330 (3776615) 3776615..3777193 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T161688 WP_000615976.1 NZ_CP055167:3774497-3774775 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T161688 NZ_CP071248:c1586151-1585849 [Bifidobacterium longum subsp. infantis]
ATCAAGCAATCCGCCGAATACCGCAAGTGGTTCAAGAAACTCAGGGACCACAAAGCGAAGGCCGCCATCCAAGCCCGGCT
CGATGCCTGCAAGCTCGCAGGCAGGCCATTCGGCGACATCAAACCCGTGGGAGGCCCGGTCAGCGAGATGCGGTTCCACA
CCGGGGCCGGATACCGCGTCTACTTCGCCATGCAGGGCAACGTGCTCATGCTGCTGCTCGCAGGCGGCGACAAAAGCACC
CAGCAGACCGACATCAGACAAGCCCACGACATCCTCAACGACTACAAGGAACAACGACAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT161688 WP_000729704.1 NZ_CP055167:3774234-3774494 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT161688 NZ_CP071248:c1585852-1585568 [Bifidobacterium longum subsp. infantis]
ATGAGCACCGAATTCACCGACTACGACACCAGCGAACACCTCGAAAACGAACAGGACATCATCGCCTACCTCAACGCCAT
AGCCGAATACGACGACCCCGCACTCATGCAGGCCGCGCTCGGCAACGTCGCCAAGGCCCGGGGCATGACCCAAATCGCCA
AGGACGCGGGCGTGGGGCGCGAAAGCCTCTACAAGAGCCTCAGCAAGGACGGCAACCCCAGCTTCCAAACCATCGCCAAG
GTAATCCACGCCCTCGGCGGACGCCTCACCATCCAAGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References