Detailed information of TA system TA161675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1086964..1087691
• Replicon: chromosome
• Accession: NZ_CP055167
• Organism: Shigella flexneri strain STEFF_24

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: HUZ50_RS05225
• Protein ID: WP_000547563.1
• Coordinates: 1086964..1087275 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUZ50_RS05230
• Protein ID: WP_000126294.1
• Coordinates: 1087272..1087691 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ50_RS05195 (1082107)	1082107..1083816	+	1710	WP_063625439.1	formate hydrogenlyase subunit HycE	-
HUZ50_RS05200 (1083826)	1083826..1084368	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
HUZ50_RS05205 (1084368)	1084368..1085135	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
HUZ50_RS05210 (1085132)	1085132..1085542	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
HUZ50_RS05215 (1085535)	1085535..1086005	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HUZ50_RS05220 (1086048)	1086048..1086791	+	744	WP_058101263.1	hypothetical protein	-
HUZ50_RS05225 (1086964)	1086964..1087275	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
HUZ50_RS05230 (1087272)	1087272..1087691	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
HUZ50_RS05235 (1087805)	1087805..1089229	-	1425	WP_000110365.1	6-phospho-beta-glucosidase AscB	-
HUZ50_RS05240 (1089238)	1089238..1090695	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HUZ50_RS05245 (1090955)	1090955..1091965	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
HUZ50_RS05250 (1092114)	1092114..1092641	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T161675 WP_000547563.1 NZ_CP055167:1086964-1087275 [Shigella flexneri]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T161675 NZ_CP071244:c4164438-4164016 [Serratia marcescens subsp. marcescens ATCC 13880]
GTGGCCCAGTGGCGATGTGATATCCGCATTTCCTGGCGCACCCAGCTGTTTTCACTGTTGACCCACGGCGTTCTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAGGGCTTTGGCCCGCTCTGGCTGGTGCTGCTGACGCTGGTGGTGTTTCAGTGCA
TTCGCAGCCAGAAGCGCATCGCCGCGGTGCAGGGTGAGCTGCGGCTGCTGGCGGACAGGCGTTTCAGCTGGCACGGCCGC
GAGTGGCGGCTGGCGAAAAAACCGTGGATGCCGGGTTACGGCATGCTGTTGACGCTGCAGCCGATGGAGGGCAAAAAGCG
CCGCCGGCTGTGGCTGGCTTCCGACTGCATGAGCAAAGAGGAGTGGCGCCACCTGCGGCAGCTGTTGCTGCATCCGCCGG
CGGGTAACGGCGAGGAACCCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT161675 WP_000126294.1 NZ_CP055167:1087272-1087691 [Shigella flexneri]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT161675 NZ_CP071244:c4164685-4164419 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGATATTAACAACAAAGCACGTATTCACTGGGCTTGCCGTCGTGGCATGCGCGAGCTGGACATCTCCATCATGCCGTT
CTTCGAATACGAATACGACAGCCTGAATGACGCGGACAAGGCGCTGTTTATCCGCCTGCTGGAGTGTGACGATCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGATGGCGAGCTGCAGAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22