Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 641211..642010 Replicon chromosome
Accession NZ_CP055167
Organism Shigella flexneri strain STEFF_24

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag HUZ50_RS03120 Protein ID WP_000347267.1
Coordinates 641211..641675 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HUZ50_RS03125 Protein ID WP_001307405.1
Coordinates 641675..642010 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ50_RS03090 (636212) 636212..636646 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HUZ50_RS03095 (636664) 636664..637542 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
HUZ50_RS03100 (637532) 637532..638311 - 780 WP_063625504.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUZ50_RS03105 (638322) 638322..638795 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUZ50_RS03110 (638818) 638818..640098 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUZ50_RS03115 (640347) 640347..641156 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUZ50_RS03120 (641211) 641211..641675 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUZ50_RS03125 (641675) 641675..642010 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUZ50_RS03130 (642159) 642159..643730 - 1572 WP_001273741.1 galactarate dehydratase -
HUZ50_RS03135 (644105) 644105..645439 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HUZ50_RS03140 (645455) 645455..646225 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T161672 WP_000347267.1 NZ_CP055167:c641675-641211 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T161672 NZ_CP071244:2039698-2039794 [Serratia marcescens subsp. marcescens ATCC 13880]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT161672 WP_001307405.1 NZ_CP055167:c642010-641675 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT161672 NZ_CP071244:c2039794-2039654 [Serratia marcescens subsp. marcescens ATCC 13880]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References